DOI: 10.1083/jcb.104.4.805. A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones

A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones

10.1083/jcb.104.4.805

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have detected and begun to characterize a 17-kD centromere-specific protein, CENP-A (Earnshaw, W. C., and N. Rothfield, 1985, Chromosoma., 91:313-321). Sera from several humans with CREST scleroderma autoimmune disease (CREST: calcinosis, Raynaud's phenomenon, esophageal dsymotility, sclerodactyly, and telangiectasia) bind this protein in immunoblot assays of HeLa whole cell or nuclear extracts. We have affinity purified the anti-17-kD centromere protein (anti-CENP-A) specific antibodies from immunoblots of HeLa nuclear protein. The antibodies react with epitopes present on CENP-A derived from humans but apparently do not recognize specific epitopes in either rat or chicken nuclei. Only human nuclear protein is CENP-A positive by immunoblot. Furthermore, human cells show localization of anti-CENP-A antibody to centromeres by immunofluorescence microscopy, whereas rat cells do not. On extraction from the nucleus, CENP-A copurifies with core histones and with nucleosome core particles. We conclude that this centromere-specific protein is a histone-like component of chromatin. The data suggest that CENP-A functions as a centromere-specific core histone.

Bibliography

Palmer, D., OâDay, K., Wener, M., Andrews, B., & Margolis, R. (1987). A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones. The Journal of Cell Biology, 104(4), 805â815.

Authors 5

DK Palmer (first)
K O'Day (additional)
MH Wener (additional)
BS Andrews (additional)
RL Margolis (additional)

References 0 Referenced 344

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8:10 p.m.)
Indexed	1 week, 3 days ago (Aug. 23, 2025, 9:33 p.m.)
Issued	38 years, 5 months ago (April 1, 1987)
Published	38 years, 5 months ago (April 1, 1987)
Published Online	38 years, 5 months ago (April 1, 1987)
Published Print	38 years, 5 months ago (April 1, 1987)

Funders 0

None

BibTeX

@article{Palmer_1987, title={A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones}, volume={104}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.104.4.805}, DOI={10.1083/jcb.104.4.805}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Palmer, DK and O’Day, K and Wener, MH and Andrews, BS and Margolis, RL}, year={1987}, month=apr, pages={805–815} }

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