DOI: 10.1083/jcb.104.2.221. A vesicular intermediate in the transport of hepatoma secretory proteins from the rough endoplasmic reticulum to the Golgi complex.

A vesicular intermediate in the transport of hepatoma secretory proteins from the rough endoplasmic reticulum to the Golgi complex.

10.1083/jcb.104.2.221

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have identified a vesicle fraction that contains alpha 1-antitrypsin and other human HepG2 hepatoma secretory proteins en route from the rough endoplasmic reticulum (RER) to the cis face of the Golgi complex. [35S]Methionine pulse-labeled cells were chased for various periods of time, and then a postnuclear supernatant fraction was resolved on a shallow sucrose-D2O gradient. This intermediate fraction has a density lighter than RER or Golgi vesicles. Most alpha 1-antitrypsin in this fraction (P1) bears N-linked oligosaccharides of composition similar to that of alpha 1-antitrypsin within the RER; mainly Man8GlcNac2 with lesser amounts of Man7GlcNac2 and Man9GlcNac2; this suggests that the protein has not yet reacted with alpha-mannosidase-I on the cis face of the Golgi complex. This light vesicle species is the first post-ER fraction to be filled by labeled alpha 1-antitrypsin after a short chase, and newly made secretory proteins enter this compartment in proportion to their rate of exit from the RER and their rate of secretion from the cells: alpha 1-antitrypsin and albumin faster than preC3 and alpha 1-antichymotrypsin, faster, in turn, then transferrin. Deoxynojirimycin, a drug that blocks removal of glucose residues from alpha 1-antitrypsin in the RER and blocks its intracellular maturation, also blocks its appearance in this intermediate compartment. Upon further chase of the cells, we detect sequential maturation of alpha 1-antitrypsin to two other intracellular forms: first, P2, a form that has the same gel mobility as P1 but that bears an endoglycosidase H-resistant oligosaccharide and is found in a compartment--probably the medial Golgi complex--of density higher than that of the intermediate that contains P1; and second, the mature sialylated form of alpha 1-antitrypsin.

Bibliography

Lodish, H. F., Kong, N., Hirani, S., & Rasmussen, J. (1987). A vesicular intermediate in the transport of hepatoma secretory proteins from the rough endoplasmic reticulum to the Golgi complex. The Journal of Cell Biology, 104(2), 221â230.

Authors 4

H F Lodish (first)
N Kong (additional)
S Hirani (additional)
J Rasmussen (additional)

References 0 Referenced 73

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8:05 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:02 p.m.)
Issued	38 years, 7 months ago (Feb. 1, 1987)
Published	38 years, 7 months ago (Feb. 1, 1987)
Published Online	38 years, 7 months ago (Feb. 1, 1987)
Published Print	38 years, 7 months ago (Feb. 1, 1987)

Funders 0

None

BibTeX

@article{Lodish_1987, title={A vesicular intermediate in the transport of hepatoma secretory proteins from the rough endoplasmic reticulum to the Golgi complex.}, volume={104}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.104.2.221}, DOI={10.1083/jcb.104.2.221}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Lodish, H F and Kong, N and Hirani, S and Rasmussen, J}, year={1987}, month=feb, pages={221–230} }

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