DOI: 10.1083/jcb.103.3.767. Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.

10.1083/jcb.103.3.767

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

An extended synthetic oligonucleotide (58-mer) has been used to identify and characterize a human liver gap junction cDNA. The cDNA is 1,574 bases long and contains the entire coding region for a gap junction protein. In vitro translation of the RNA products of this cDNA is consistent with it coding for a 32,022-D protein. Southern blot analysis indicates that the gap junction gene is present as a single copy, and that it can be detected in a variety of organisms using the human liver cDNA as a probe. The human cDNA has been used to screen a rat liver cDNA library, and a rat liver junction cDNA clone has been isolated. The rat liver clone is 1,127 bases in length, and it has strong sequence homology to the human cDNA in the protein-coding region, but less extensive homology in the 3'-untranslated region.

Bibliography

Kumar, N. M., & Gilula, N. B. (1986). Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein. The Journal of Cell Biology, 103(3), 767â776.

Authors 2

N M Kumar (first)
N B Gilula (additional)

References 0 Referenced 315

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8 p.m.)
Indexed	1 week, 4 days ago (Aug. 23, 2025, 1 a.m.)
Issued	39 years ago (Sept. 1, 1986)
Published	39 years ago (Sept. 1, 1986)
Published Online	39 years ago (Sept. 1, 1986)
Published Print	39 years ago (Sept. 1, 1986)

Funders 0

None

BibTeX

@article{Kumar_1986, title={Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.}, volume={103}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.103.3.767}, DOI={10.1083/jcb.103.3.767}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Kumar, N M and Gilula, N B}, year={1986}, month=sep, pages={767–776} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        23
      ]
    ],
    "date-time": "2025-08-23T05:00:26Z",
    "timestamp": 1755925226696
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "3",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1986,
        9,
        1
      ]
    ]
  },
  "abstract": "<jats:p>An extended synthetic oligonucleotide (58-mer) has been used to identify and characterize a human liver gap junction cDNA. The cDNA is 1,574 bases long and contains the entire coding region for a gap junction protein. In vitro translation of the RNA products of this cDNA is consistent with it coding for a 32,022-D protein. Southern blot analysis indicates that the gap junction gene is present as a single copy, and that it can be detected in a variety of organisms using the human liver cDNA as a probe. The human cDNA has been used to screen a rat liver cDNA library, and a rat liver junction cDNA clone has been isolated. The rat liver clone is 1,127 bases in length, and it has strong sequence homology to the human cDNA in the protein-coding region, but less extensive homology in the 3'-untranslated region.</jats:p>",
  "DOI": "10.1083/jcb.103.3.767",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:18:20Z",
    "timestamp": 1084580300000
  },
  "page": "767-776",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 315,
  "title": "Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.",
  "prefix": "10.1083",
  "volume": "103",
  "author": [
    {
      "given": "N M",
      "family": "Kumar",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "N B",
      "family": "Gilula",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1986,
        9,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/103/3/767/1459442/767.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/103/3/767/1459442/767.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T00:00:27Z",
    "timestamp": 1689984027000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/103/3/767/13440/Cloning-and-characterization-of-human-and-rat"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1986,
        9,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1986,
          9,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.103.3.767",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1986,
        9,
        1
      ]
    ]
  }
}