DOI: 10.1083/jcb.102.5.1679. Aster-free spindle poles in insect spermatocytes: evidence for chromosome-induced spindle formation?

Aster-free spindle poles in insect spermatocytes: evidence for chromosome-induced spindle formation?

10.1083/jcb.102.5.1679

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Tipulid spermatocytes form normally functioning bipolar spindles after one of the centrosomes is experimentally dislocated from the nucleus in late diakinesis (Dietz, R., 1959, Z. Naturforsch., 14b:749-752; Dietz, R., 1963, Zool. Anz. Suppl., 23:131-138; Dietz, R., 1966, Heredity, 19:161-166). The possibility that dissociated pericentriolar material (PCM) is nevertheless responsible for the formation of the spindle in these cells cannot be ruled out based on live observation. In studying serial sections of complete cells and of lysed cells, it was found that centrosome-free spindle poles in the crane fly show neither pericentriolar-like material nor aster microtubules, whereas the displaced centrosomes appear complete, i.e., consist of a centriole pair, aster microtubules, and PCM. Exposure to a lysis buffer containing tubulin resulted in an increase of centrosomal asters due to aster microtubule polymerization. Aster-free spindle poles did not show any reaction, also indicating the absence of PCM at these poles. The results favor the hypothesis of chromosome-induced spindle pole formation at the onset of prometaphase and the dispensability of PCM in Pales.

Bibliography

Steffen, W., Fuge, H., Dietz, R., Bastmeyer, M., & MÃ¼ller, G. (1986). Aster-free spindle poles in insect spermatocytes: evidence for chromosome-induced spindle formation? The Journal of Cell Biology, 102(5), 1679â1687.

Authors 5

W Steffen (first)
H Fuge (additional)
R Dietz (additional)
M Bastmeyer (additional)
G Müller (additional)

References 0 Referenced 73

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:19 p.m.)
Indexed	1 year, 3 months ago (May 6, 2024, 12:33 p.m.)
Issued	39 years, 4 months ago (May 1, 1986)
Published	39 years, 4 months ago (May 1, 1986)
Published Online	39 years, 4 months ago (May 1, 1986)
Published Print	39 years, 4 months ago (May 1, 1986)

Funders 0

None

BibTeX

@article{Steffen_1986, title={Aster-free spindle poles in insect spermatocytes: evidence for chromosome-induced spindle formation?}, volume={102}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.102.5.1679}, DOI={10.1083/jcb.102.5.1679}, number={5}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Steffen, W and Fuge, H and Dietz, R and Bastmeyer, M and Müller, G}, year={1986}, month=may, pages={1679–1687} }

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