DOI: 10.1083/jcb.102.5.1543. Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.

Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.

10.1083/jcb.102.5.1543

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We describe an in vitro system with all components derived from the yeast Saccharomyces cerevisiae that can translocate a yeast secretory protein across microsomal membranes. In vitro transcribed prepro-alpha-factor mRNA served to program a membrane-depleted yeast translation system. Translocation and core glycosylation of prepro-alpha-factor were observed when yeast microsomal membranes were added during or after translation. A membrane potential is not required for translocation. However, ATP is required for translocation and nonhydrolyzable analogues of ATP cannot serve as a substitute. These findings suggest that ATP hydrolysis may supply the energy required for translocation of proteins across the endoplasmic reticulum.

Bibliography

Waters, M. G., & Blobel, G. (1986). Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis. The Journal of Cell Biology, 102(5), 1543â1550.

Authors 2

M G Waters (first)
G Blobel (additional)

References 0 Referenced 220

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 7:27 p.m.)
Indexed	3 months, 3 weeks ago (May 1, 2025, 12:21 a.m.)
Issued	39 years, 3 months ago (May 1, 1986)
Published	39 years, 3 months ago (May 1, 1986)
Published Online	39 years, 3 months ago (May 1, 1986)
Published Print	39 years, 3 months ago (May 1, 1986)

Funders 0

None

BibTeX

@article{Waters_1986, title={Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.}, volume={102}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.102.5.1543}, DOI={10.1083/jcb.102.5.1543}, number={5}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Waters, M G and Blobel, G}, year={1986}, month=may, pages={1543–1550} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        1
      ]
    ],
    "date-time": "2025-05-01T04:21:26Z",
    "timestamp": 1746073286625
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "5",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1986,
        5,
        1
      ]
    ]
  },
  "abstract": "<jats:p>We describe an in vitro system with all components derived from the yeast Saccharomyces cerevisiae that can translocate a yeast secretory protein across microsomal membranes. In vitro transcribed prepro-alpha-factor mRNA served to program a membrane-depleted yeast translation system. Translocation and core glycosylation of prepro-alpha-factor were observed when yeast microsomal membranes were added during or after translation. A membrane potential is not required for translocation. However, ATP is required for translocation and nonhydrolyzable analogues of ATP cannot serve as a substitute. These findings suggest that ATP hydrolysis may supply the energy required for translocation of proteins across the endoplasmic reticulum.</jats:p>",
  "DOI": "10.1083/jcb.102.5.1543",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:18:20Z",
    "timestamp": 1084580300000
  },
  "page": "1543-1550",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 220,
  "title": "Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.",
  "prefix": "10.1083",
  "volume": "102",
  "author": [
    {
      "given": "M G",
      "family": "Waters",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "G",
      "family": "Blobel",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1986,
        5,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/102/5/1543/1459145/1543.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/102/5/1543/1459145/1543.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        21
      ]
    ],
    "date-time": "2023-07-21T23:27:52Z",
    "timestamp": 1689982072000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/102/5/1543/55185/Secretory-protein-translocation-in-a-yeast-cell"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1986,
        5,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1986,
          5,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.102.5.1543",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1986,
        5,
        1
      ]
    ]
  }
}