DOI: 10.1083/jcb.101.3.1094. Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.

Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.

10.1083/jcb.101.3.1094

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

The bacteriophage SP6 promoter and RNA polymerase were used to synthesize sense and antisense RNAs coding for the enzymes thymidine kinase (TK) and chloramphenicol acetyl transferase (CAT). Injection of antisense CAT RNA into frog oocytes inhibited expression of sense CAT mRNA. Similarly, antisense TK RNA inhibited expression of sense TK mRNA. Antisense RNAs were stable in oocytes and had no detectable effect on either the expression of endogenous proteins or on the expression of nonhomologous RNA transcripts. CAT activity expressed from a plasmid transcribed in the oocyte nucleus was also inhibited by antisense RNA injected into the oocyte cytoplasm. The data suggest that antisense RNA will be useful in identifying the function of specific mRNA sequences during early development of the frog.

Bibliography

Harland, R., & Weintraub, H. (1985). Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA. The Journal of Cell Biology, 101(3), 1094â1099.

Authors 2

R Harland (first)
H Weintraub (additional)

References 0 Referenced 144

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 7:18 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 3, 2024, 4:53 a.m.)
Issued	39 years, 11 months ago (Sept. 1, 1985)
Published	39 years, 11 months ago (Sept. 1, 1985)
Published Online	39 years, 11 months ago (Sept. 1, 1985)
Published Print	39 years, 11 months ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{Harland_1985, title={Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.}, volume={101}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.101.3.1094}, DOI={10.1083/jcb.101.3.1094}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Harland, R and Weintraub, H}, year={1985}, month=sep, pages={1094–1099} }

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