DOI: 10.1083/jcb.100.5.1706. Topoisomerase II is a structural component of mitotic chromosome scaffolds.

Topoisomerase II is a structural component of mitotic chromosome scaffolds.

10.1083/jcb.100.5.1706

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase II, an abundant nuclear enzyme that controls DNA topological states: anti-scaffold antibody inhibits the strand-passing activity of DNA topoisomerase II; and both anti-scaffold antibody and an independent antibody raised against purified bovine topoisomerase II recognize identical partial proteolysis fragments of the 170,000-mol-wt scaffold protein in immunoblots. Our results suggest that topoisomerase II may be an enzyme that is also a structural protein of interphase nuclei and mitotic chromosomes.

Bibliography

Earnshaw, W. C., Halligan, B., Cooke, C. A., Heck, M. M., & Liu, L. F. (1985). Topoisomerase II is a structural component of mitotic chromosome scaffolds. The Journal of Cell Biology, 100(5), 1706â1715.

Authors 5

W C Earnshaw (first)
B Halligan (additional)
C A Cooke (additional)
M M Heck (additional)
L F Liu (additional)

References 0 Referenced 552

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 7:11 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 6, 2025, 9:07 a.m.)
Issued	40 years, 3 months ago (May 1, 1985)
Published	40 years, 3 months ago (May 1, 1985)
Published Online	40 years, 3 months ago (May 1, 1985)
Published Print	40 years, 3 months ago (May 1, 1985)

Funders 0

None

BibTeX

@article{Earnshaw_1985, title={Topoisomerase II is a structural component of mitotic chromosome scaffolds.}, volume={100}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.100.5.1706}, DOI={10.1083/jcb.100.5.1706}, number={5}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Earnshaw, W C and Halligan, B and Cooke, C A and Heck, M M and Liu, L F}, year={1985}, month=may, pages={1706–1715} }

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