Abstract
The difficulties in sectioning frozen biological objects for electron microscopic investigations are overcome by Steere's freezing-etching method. In order to test this method and to open up a wide field of application, the new freezing-ultramicrotome has been designed. The apparatus consists of the combination of an ultramicrotome with freezing-drying and shadow-casting installations in the same vacuum container. The preliminary results show, on the one hand, the practicability of all preparational steps and, on the other, that it is possible to resolve internal structures of cell organelles and even macromolecular patterns.
Dates
Type | When |
---|---|
Created | 21 years, 3 months ago (May 14, 2004, 1:40 p.m.) |
Deposited | 2 years, 1 month ago (July 21, 2023, 7:04 p.m.) |
Indexed | 3 weeks, 2 days ago (Aug. 2, 2025, 1:04 a.m.) |
Issued | 64 years, 3 months ago (May 1, 1961) |
Published | 64 years, 3 months ago (May 1, 1961) |
Published Online | 64 years, 3 months ago (May 1, 1961) |
Published Print | 64 years, 3 months ago (May 1, 1961) |
@article{Moor_1961, title={A NEW FREEZING-ULTRAMICROTOME}, volume={10}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.10.1.1}, DOI={10.1083/jcb.10.1.1}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Moor, H. and Mühlethaler, K. and Waldner, H. and Frey-Wyssling, A.}, year={1961}, month=may, pages={1–13} }