DOI: 10.1074/mcp.m500084-mcp200. Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics

Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics

10.1074/mcp.m500084-mcp200

Crossref journal-article

Elsevier BV

Molecular & Cellular Proteomics (78)

Bibliography

Old, W. M., Meyer-Arendt, K., Aveline-Wolf, L., Pierce, K. G., Mendoza, A., Sevinsky, J. R., Resing, K. A., & Ahn, N. G. (2005). Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics. Molecular & Cellular Proteomics, 4(10), 1487â1502.

Authors 8

William M. Old (first)
Karen Meyer-Arendt (additional)
Lauren Aveline-Wolf (additional)
Kevin G. Pierce (additional)
Alex Mendoza (additional)
Joel R. Sevinsky (additional)
Katheryn A. Resing (additional)
Natalie G. Ahn (additional)

References 38 Referenced 1,005

10.1021/ac960799q / Anal. Chem. / Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level by McCormack (1997)
10.1038/10890 / Nat. Biotechnol. / Direct analysis of protein complexes using mass spectrometry by Link (1999)
10.1021/ac010617e / Anal. Chem. / An automated multidimensional protein identification technology for shotgun proteomics by Wolters (2001)
10.1021/ac00104a020 / Anal. Chem. / Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database by Yates (1995)
10.1016/1044-0305(94)80016-2 / J. Am. Soc. Mass Spectrom. / An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database by Eng (1994)
10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2 / Electrophoresis / Probability-based protein identification by searching sequence databases using mass spectrometry data by Perkins (1999)
10.1038/13690 / Nat. Biotechnol. / Quantitative analysis of complex protein mixtures using isotope-coded affinity tags by Gygi (1999)
10.1038/nbt0502-512 / Nat. Biotechnol. / Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry by Zhou (2002)
10.1021/ac001404c / Anal. Chem. / Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus by Yao (2001)
10.1016/j.jasms.2004.04.034 / J. Am. Soc. Mass Spectrom. / Charge competition and the linear dynamic range of detection in electrospray ionization mass spectrometry by Tang (2004)
10.1074/mcp.M200001-MCP200 / Mol. Cell. Proteomics / Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae by Griffin (2002)
10.1002/pmic.200400994 / Proteomics / Large-scale evaluation of quantitative reproducibility and proteome coverage using acid cleavable isotope coded affinity tag mass spectrometry for proteomic profiling by Molloy (2005)
10.1021/pr015509n / J. Proteome Res. / Proteome analysis of low-abundance proteins using multidimensional chromatography and isotope-coded affinity tags by Gygi (2002)
10.1021/ac026127j / Anal. Chem. / Abundance ratio-dependent proteomic analysis by mass spectrometry by Griffin (2003)
10.1021/ac0256991 / Anal. Chem. / Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatography-tandem mass spectrometry by Bondarenko (2002)
10.1021/pr025517j / J. Proteome Res. / Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry by Chelius (2002)
10.1021/ac026468x / Anal. Chem. / Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards by Wang (2003)
10.1021/ac0498563 / Anal. Chem. / A model for random sampling and estimation of relative protein abundance in shotgun proteomics by Liu (2004)
10.1038/nature02046 / Nature / Global analysis of protein expression in yeast by Ghaemmaghami (2003)
10.1021/ac035229m / Anal. Chem. / Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics by Resing (2004)
10.1128/MCB.17.4.1947 / Mol. Cell. Biol. / Megakaryocytic differentiation induced by constitutive activation of mitogen-activated protein kinase kinase by Whalen (1997)
10.1128/MCB.24.10.4534-4545.2004 / Mol. Cell. Biol. / Extracellular signal-regulated kinase induces the megakaryocyte GPIIb/CD41 gene through MafB/Kreisler by Sevinsky (2004)
10.1016/S1097-2765(00)00132-5 / Mol. Cell / Identification of novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry by Lewis (2000)
10.1093/biostatistics/4.2.249 / Biostatistics / Exploration, normalization, and summaries of high density oligonucleotide array probe level data by Irizarry (2003)
10.1186/gb-2004-5-10-r80 / Genome Biol. / Bioconductor: open software development for computational biology and bioinformatics by Gentleman (2004)
10.1093/bioinformatics/bth449 / Bioinformatics / limmaGUI: agraphical user interface for linear modeling of microarray data by Wettenhall (2004)
10.1002/1522-2683(200209)23:18<3205::AID-ELPS3205>3.0.CO;2-Y / Electrophoresis / Approaching complete peroxisome characterization by gas-phase fractionation by Yi (2002)
10.1002/1615-9861(200101)1:1<93::AID-PROT93>3.0.CO;2-3 / Proteomics / Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry. I. Profiling an unfractionated tryptic digest by Spahr (2001)
10.1002/1615-9861(200101)1:1<108::AID-PROT108>3.0.CO;2-5 / Proteomics / Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry. II. Limitations of complex mixture analyses by Davis (2001)
10.1002/pmic.200300721 / Proteomics / The International Protein Index: an integrated database for proteomics experiments by Kersey (2004)
10.1021/pr049920x / J. Proteome Res. / DBParser: web-based software for shotgun proteomic data analyses by Yang (2004)
10.1021/ac034633i / Anal. Chem. / Automated statistical analysis of protein abundance ratios from data generated by stable-isotope dilution and tandem mass spectrometry by Li (2003)
10.1021/ac034790h / Anal. Chem. / A correlation algorithm for the automated quantitative analysis of shotgun proteomics data by MacCoss (2003)
10.1021/ac00002a010 / Anal. Chem. / Statistical treatment for rejection of deviant values: critical values of Dixon’s “Q” parameter and related subrange ratios at the 95% confidence level by Rorabacher (1991)
10.1093/nar/gng052 / Nucleic Acids Res. / Testing for differentially expressed genes with microarray data by Tsai (2003)
{'key': '10.1074/mcp.M500084-MCP200_bib36', 'series-title': 'Biometry: the Principles and Practice of Statistics in Biological Research', 'first-page': '729', 'author': 'Sokal', 'year': '1995'} / Biometry: the Principles and Practice of Statistics in Biological Research by Sokal (1995)
10.1093/bioinformatics/bth924 / Bioinformatics / Statisticalmodeling of sequencing errors in SAGE libraries by Beissbarth (2004)
10.1093/bioinformatics/bth446 / Bioinformatics / Quantifying reproducibility for differential proteomics: noise analysis for protein liquid chromatography-mass spectrometry of human serum by Anderle (2004)

Dates

Type	When
Created	20 years, 2 months ago (June 24, 2005, 8:12 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 5:18 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:55 p.m.)
Issued	19 years, 10 months ago (Oct. 1, 2005)
Published	19 years, 10 months ago (Oct. 1, 2005)
Published Print	19 years, 10 months ago (Oct. 1, 2005)

Funders 0

None

BibTeX

@article{Old_2005, title={Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics}, volume={4}, ISSN={1535-9476}, url={http://dx.doi.org/10.1074/mcp.m500084-mcp200}, DOI={10.1074/mcp.m500084-mcp200}, number={10}, journal={Molecular & Cellular Proteomics}, publisher={Elsevier BV}, author={Old, William M. and Meyer-Arendt, Karen and Aveline-Wolf, Lauren and Pierce, Kevin G. and Mendoza, Alex and Sevinsky, Joel R. and Resing, Katheryn A. and Ahn, Natalie G.}, year={2005}, month=oct, pages={1487–1502} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T20:55:07Z",
    "timestamp": 1754600107127
  },
  "reference-count": 38,
  "publisher": "Elsevier BV",
  "issue": "10",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            10,
            1
          ]
        ],
        "date-time": "2005-10-01T00:00:00Z",
        "timestamp": 1128124800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2020,
            8,
            19
          ]
        ],
        "date-time": "2020-08-19T00:00:00Z",
        "timestamp": 1597795200000
      },
      "content-version": "vor",
      "delay-in-days": 5436,
      "URL": "http://creativecommons.org/licenses/by/4.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2005,
        10
      ]
    ]
  },
  "DOI": "10.1074/mcp.m500084-mcp200",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        6,
        25
      ]
    ],
    "date-time": "2005-06-25T00:12:57Z",
    "timestamp": 1119658377000
  },
  "page": "1487-1502",
  "source": "Crossref",
  "is-referenced-by-count": 1005,
  "title": "Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics",
  "prefix": "10.1016",
  "volume": "4",
  "author": [
    {
      "given": "William M.",
      "family": "Old",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Karen",
      "family": "Meyer-Arendt",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Lauren",
      "family": "Aveline-Wolf",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Kevin G.",
      "family": "Pierce",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Alex",
      "family": "Mendoza",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Joel R.",
      "family": "Sevinsky",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Katheryn A.",
      "family": "Resing",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Natalie G.",
      "family": "Ahn",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1074/mcp.M500084-MCP200_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "767",
      "DOI": "10.1021/ac960799q",
      "article-title": "Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level",
      "volume": "69",
      "author": "McCormack",
      "year": "1997",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "676",
      "DOI": "10.1038/10890",
      "article-title": "Direct analysis of protein complexes using mass spectrometry",
      "volume": "17",
      "author": "Link",
      "year": "1999",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "5683",
      "DOI": "10.1021/ac010617e",
      "article-title": "An automated multidimensional protein identification technology for shotgun proteomics",
      "volume": "73",
      "author": "Wolters",
      "year": "2001",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "1426",
      "DOI": "10.1021/ac00104a020",
      "article-title": "Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database",
      "volume": "67",
      "author": "Yates",
      "year": "1995",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "976",
      "DOI": "10.1016/1044-0305(94)80016-2",
      "article-title": "An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database",
      "volume": "5",
      "author": "Eng",
      "year": "1994",
      "journal-title": "J. Am. Soc. Mass Spectrom."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "3551",
      "DOI": "10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2",
      "article-title": "Probability-based protein identification by searching sequence databases using mass spectrometry data",
      "volume": "20",
      "author": "Perkins",
      "year": "1999",
      "journal-title": "Electrophoresis"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "994",
      "DOI": "10.1038/13690",
      "article-title": "Quantitative analysis of complex protein mixtures using isotope-coded affinity tags",
      "volume": "17",
      "author": "Gygi",
      "year": "1999",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "512",
      "DOI": "10.1038/nbt0502-512",
      "article-title": "Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry",
      "volume": "20",
      "author": "Zhou",
      "year": "2002",
      "journal-title": "Nat. Biotechnol."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "2836",
      "DOI": "10.1021/ac001404c",
      "article-title": "Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus",
      "volume": "73",
      "author": "Yao",
      "year": "2001",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "1416",
      "DOI": "10.1016/j.jasms.2004.04.034",
      "article-title": "Charge competition and the linear dynamic range of detection in electrospray ionization mass spectrometry",
      "volume": "15",
      "author": "Tang",
      "year": "2004",
      "journal-title": "J. Am. Soc. Mass Spectrom."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "323",
      "DOI": "10.1074/mcp.M200001-MCP200",
      "article-title": "Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae",
      "volume": "1",
      "author": "Griffin",
      "year": "2002",
      "journal-title": "Mol. Cell. Proteomics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "1204",
      "DOI": "10.1002/pmic.200400994",
      "article-title": "Large-scale evaluation of quantitative reproducibility and proteome coverage using acid cleavable isotope coded affinity tag mass spectrometry for proteomic profiling",
      "volume": "5",
      "author": "Molloy",
      "year": "2005",
      "journal-title": "Proteomics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1021/pr015509n",
      "article-title": "Proteome analysis of low-abundance proteins using multidimensional chromatography and isotope-coded affinity tags",
      "volume": "1",
      "author": "Gygi",
      "year": "2002",
      "journal-title": "J. Proteome Res."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "867",
      "DOI": "10.1021/ac026127j",
      "article-title": "Abundance ratio-dependent proteomic analysis by mass spectrometry",
      "volume": "75",
      "author": "Griffin",
      "year": "2003",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "4741",
      "DOI": "10.1021/ac0256991",
      "article-title": "Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatography-tandem mass spectrometry",
      "volume": "74",
      "author": "Bondarenko",
      "year": "2002",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1021/pr025517j",
      "article-title": "Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry",
      "volume": "1",
      "author": "Chelius",
      "year": "2002",
      "journal-title": "J. Proteome Res."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "4818",
      "DOI": "10.1021/ac026468x",
      "article-title": "Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards",
      "volume": "75",
      "author": "Wang",
      "year": "2003",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "4193",
      "DOI": "10.1021/ac0498563",
      "article-title": "A model for random sampling and estimation of relative protein abundance in shotgun proteomics",
      "volume": "76",
      "author": "Liu",
      "year": "2004",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "737",
      "DOI": "10.1038/nature02046",
      "article-title": "Global analysis of protein expression in yeast",
      "volume": "425",
      "author": "Ghaemmaghami",
      "year": "2003",
      "journal-title": "Nature"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib20",
      "doi-asserted-by": "crossref",
      "first-page": "3556",
      "DOI": "10.1021/ac035229m",
      "article-title": "Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics",
      "volume": "76",
      "author": "Resing",
      "year": "2004",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "1947",
      "DOI": "10.1128/MCB.17.4.1947",
      "article-title": "Megakaryocytic differentiation induced by constitutive activation of mitogen-activated protein kinase kinase",
      "volume": "17",
      "author": "Whalen",
      "year": "1997",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "4534",
      "DOI": "10.1128/MCB.24.10.4534-4545.2004",
      "article-title": "Extracellular signal-regulated kinase induces the megakaryocyte GPIIb/CD41 gene through MafB/Kreisler",
      "volume": "24",
      "author": "Sevinsky",
      "year": "2004",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "1343",
      "DOI": "10.1016/S1097-2765(00)00132-5",
      "article-title": "Identification of novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry",
      "volume": "6",
      "author": "Lewis",
      "year": "2000",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1093/biostatistics/4.2.249",
      "article-title": "Exploration, normalization, and summaries of high density oligonucleotide array probe level data",
      "volume": "4",
      "author": "Irizarry",
      "year": "2003",
      "journal-title": "Biostatistics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "R80.1",
      "DOI": "10.1186/gb-2004-5-10-r80",
      "article-title": "Bioconductor: open software development for computational biology and bioinformatics",
      "volume": "5",
      "author": "Gentleman",
      "year": "2004",
      "journal-title": "Genome Biol."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "3705",
      "DOI": "10.1093/bioinformatics/bth449",
      "article-title": "limmaGUI: agraphical user interface for linear modeling of microarray data",
      "volume": "20",
      "author": "Wettenhall",
      "year": "2004",
      "journal-title": "Bioinformatics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "3205",
      "DOI": "10.1002/1522-2683(200209)23:18<3205::AID-ELPS3205>3.0.CO;2-Y",
      "article-title": "Approaching complete peroxisome characterization by gas-phase fractionation",
      "volume": "23",
      "author": "Yi",
      "year": "2002",
      "journal-title": "Electrophoresis"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "93",
      "DOI": "10.1002/1615-9861(200101)1:1<93::AID-PROT93>3.0.CO;2-3",
      "article-title": "Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry. I. Profiling an unfractionated tryptic digest",
      "volume": "1",
      "author": "Spahr",
      "year": "2001",
      "journal-title": "Proteomics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib29",
      "doi-asserted-by": "crossref",
      "first-page": "108",
      "DOI": "10.1002/1615-9861(200101)1:1<108::AID-PROT108>3.0.CO;2-5",
      "article-title": "Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry. II. Limitations of complex mixture analyses",
      "volume": "1",
      "author": "Davis",
      "year": "2001",
      "journal-title": "Proteomics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib30",
      "doi-asserted-by": "crossref",
      "first-page": "1985",
      "DOI": "10.1002/pmic.200300721",
      "article-title": "The International Protein Index: an integrated database for proteomics experiments",
      "volume": "4",
      "author": "Kersey",
      "year": "2004",
      "journal-title": "Proteomics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "1002",
      "DOI": "10.1021/pr049920x",
      "article-title": "DBParser: web-based software for shotgun proteomic data analyses",
      "volume": "3",
      "author": "Yang",
      "year": "2004",
      "journal-title": "J. Proteome Res."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "6648",
      "DOI": "10.1021/ac034633i",
      "article-title": "Automated statistical analysis of protein abundance ratios from data generated by stable-isotope dilution and tandem mass spectrometry",
      "volume": "75",
      "author": "Li",
      "year": "2003",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "6912",
      "DOI": "10.1021/ac034790h",
      "article-title": "A correlation algorithm for the automated quantitative analysis of shotgun proteomics data",
      "volume": "75",
      "author": "MacCoss",
      "year": "2003",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "139",
      "DOI": "10.1021/ac00002a010",
      "article-title": "Statistical treatment for rejection of deviant values: critical values of Dixon\u2019s \u201cQ\u201d parameter and related subrange ratios at the 95% confidence level",
      "volume": "63",
      "author": "Rorabacher",
      "year": "1991",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "e52",
      "DOI": "10.1093/nar/gng052",
      "article-title": "Testing for differentially expressed genes with microarray data",
      "volume": "31",
      "author": "Tsai",
      "year": "2003",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib36",
      "series-title": "Biometry: the Principles and Practice of Statistics in Biological Research",
      "first-page": "729",
      "author": "Sokal",
      "year": "1995"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib37",
      "doi-asserted-by": "crossref",
      "first-page": "i31",
      "DOI": "10.1093/bioinformatics/bth924",
      "article-title": "Statisticalmodeling of sequencing errors in SAGE libraries",
      "volume": "20",
      "author": "Beissbarth",
      "year": "2004",
      "journal-title": "Bioinformatics"
    },
    {
      "key": "10.1074/mcp.M500084-MCP200_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "3575",
      "DOI": "10.1093/bioinformatics/bth446",
      "article-title": "Quantifying reproducibility for differential proteomics: noise analysis for protein liquid chromatography-mass spectrometry of human serum",
      "volume": "20",
      "author": "Anderle",
      "year": "2004",
      "journal-title": "Bioinformatics"
    }
  ],
  "container-title": "Molecular &amp; Cellular Proteomics",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S1535947620300670?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S1535947620300670?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1074/mcp.M500084-MCP200",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T21:18:05Z",
    "timestamp": 1627593485000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S1535947620300670"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2005,
        10
      ]
    ]
  },
  "references-count": 38,
  "journal-issue": {
    "issue": "10",
    "published-print": {
      "date-parts": [
        [
          2005,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "S1535947620300670"
  ],
  "URL": "http://dx.doi.org/10.1074/mcp.M500084-MCP200",
  "relation": {},
  "ISSN": [
    "1535-9476"
  ],
  "subject": [],
  "container-title-short": "Molecular &amp; Cellular Proteomics",
  "published": {
    "date-parts": [
      [
        2005,
        10
      ]
    ]
  }
}