DOI: 10.1073/pnas.98.4.2053. <i>Arabidopsis genomes uncoupled 5</i> ( <i>GUN5</i> ) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction

Arabidopsis genomes uncoupled 5 ( GUN5 ) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction

10.1073/pnas.98.4.2053

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A plastid-derived signal plays an important role in the coordinated expression of both nuclear- and chloroplast-localized genes that encode photosynthesis-related proteins. Arabidopsis GUN ( g enomes un coupled) loci have been identified as components of plastid-to-nucleus signal transduction. Unlike wild-type plants, gun mutants have nuclear Lhcb1 expression in the absence of chloroplast development. We observed a synergistic phenotype in some gun double-mutant combinations, suggesting there are at least two independent pathways in plastid-to-nucleus signal transduction. There is a reduction of chlorophyll accumulation in gun4 and gun5 mutant plants, and a gun4gun5 double mutant shows an albino phenotype. We cloned the GUN5 gene, which encodes the ChlH subunit of Mg-chelatase. We also show that gun2 and gun3 are alleles of the known photomorphogenic mutants, hy1 and hy2 , which are required for phytochromobilin synthesis from heme. These findings suggest that certain perturbations of the tetrapyrrole biosynthetic pathway generate a signal from chloroplasts that causes transcriptional repression of nuclear genes encoding plastid-localized proteins. The comparison of mutant phenotypes of gun5 and another Mg-chelatase subunit (ChlI) mutant suggests a specific function for ChlH protein in the plastid-signaling pathway.

Bibliography

Mochizuki, N., Brusslan, J. A., Larkin, R., Nagatani, A., & Chory, J. (2001). Arabidopsis genomes uncoupled 5 ( GUN5 ) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction. Proceedings of the National Academy of Sciences, 98(4), 2053â2058.

Authors 5

Nobuyoshi Mochizuki (first)
Judy A. Brusslan (additional)
Robert Larkin (additional)
Akira Nagatani (additional)
Joanne Chory (additional)

References 49 Referenced 545

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:35 p.m.)
Indexed	11 hours, 38 minutes ago (Aug. 29, 2025, 6:33 a.m.)
Issued	24 years, 6 months ago (Feb. 13, 2001)
Published	24 years, 6 months ago (Feb. 13, 2001)
Published Online	24 years, 6 months ago (Feb. 13, 2001)
Published Print	24 years, 6 months ago (Feb. 13, 2001)

Funders 0

None

BibTeX

@article{Mochizuki_2001, title={Arabidopsis genomes uncoupled 5 ( GUN5 ) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.98.4.2053}, DOI={10.1073/pnas.98.4.2053}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mochizuki, Nobuyoshi and Brusslan, Judy A. and Larkin, Robert and Nagatani, Akira and Chory, Joanne}, year={2001}, month=feb, pages={2053–2058} }

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