DOI: 10.1073/pnas.98.4.1495. Ash1p is a site-specific DNA-binding protein that actively represses transcription

Ash1p is a site-specific DNA-binding protein that actively represses transcription

10.1073/pnas.98.4.1495

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

ASH1 encodes a protein that is localized specifically to the daughter cell nucleus, where it has been proposed to repress transcription of the HO gene. Using Ash1p purified from baculovirus-infected insect cells, we have shown that Ash1p binds specific DNA sequences in the HO promoter. DNase I protection analyses showed that Ash1p recognizes a consensus sequence, YTGAT. Mutation of this consensus abolishes Ash1p DNA binding in vitro . We have shown that Ash1p requires an intact zinc-binding domain in its C terminus for repression of HO in vivo and that this domain may be involved in DNA binding. A heterologous DNA-binding domain fused to an N-terminal segment of Ash1p functions as an active repressor of transcription. Our studies indicate that Ash1p is a DNA-binding protein of the GATA family with a separable transcriptional repression domain.

Bibliography

Maxon, M. E., & Herskowitz, I. (2001). Ash1p is a site-specific DNA-binding protein that actively represses transcription. Proceedings of the National Academy of Sciences, 98(4), 1495â1500.

Authors 2

Mary E. Maxon (first)
Ira Herskowitz (additional)

References 35 Referenced 38

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:34 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 1:51 p.m.)
Issued	24 years, 6 months ago (Feb. 13, 2001)
Published	24 years, 6 months ago (Feb. 13, 2001)
Published Online	24 years, 6 months ago (Feb. 13, 2001)
Published Print	24 years, 6 months ago (Feb. 13, 2001)

Funders 0

None

BibTeX

@article{Maxon_2001, title={Ash1p is a site-specific DNA-binding protein that actively represses transcription}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.98.4.1495}, DOI={10.1073/pnas.98.4.1495}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Maxon, Mary E. and Herskowitz, Ira}, year={2001}, month=feb, pages={1495–1500} }

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