DOI: 10.1073/pnas.98.2.581. High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

10.1073/pnas.98.2.581

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We describe here a system for the rapid identification, assay development, and characterization of gene-based single nucleotide polymorphisms (SNPs). This system couples informatics tools that mine candidate SNPs from public expressed sequence tag resources and automatically designs assay reagents with detection by a chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform. As a proof of concept of this system, a genomewide collection of reagents for 9,115 gene-based SNP genetic markers was rapidly developed and validated. These data provide preliminary insights into patterns of polymorphism in a genomewide collection of gene-based polymorphisms.

Bibliography

Buetow, K. H., Edmonson, M., MacDonald, R., Clifford, R., Yip, P., Kelley, J., Little, D. P., Strausberg, R., Koester, H., Cantor, C. R., & Braun, A. (2001). High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Proceedings of the National Academy of Sciences, 98(2), 581â584.

Authors 11

Kenneth H. Buetow (first)
Michael Edmonson (additional)
Richard MacDonald (additional)
Robert Clifford (additional)
Ping Yip (additional)
Jenny Kelley (additional)
Daniel P. Little (additional)
Robert Strausberg (additional)
Hubert Koester (additional)
Charles R. Cantor (additional)
Andreas Braun (additional)

References 11 Referenced 316

10.1126/science.273.5281.1516
10.1038/6851
10.1126/science.274.5287.540
10.1101/gr.6.9.807
10.1093/clinchem/43.7.1151
D P Little, A Braun, B Darnhofer-Demar, H Koster Eur J Clin Chem Clin Biochem 35, 545–548 (1997). / Eur J Clin Chem Clin Biochem by Little D P (1997)
10.1038/nm1297-1413
10.1056/NEJM199801083380202
10.1002/(SICI)1097-0231(199611)10:14<1792::AID-RCM753>3.0.CO;2-N
10.1016/0888-7543(90)90491-C
10.1126/science.282.5389.744

Dates

Type	When
Created	13 years, 1 month ago (July 26, 2012, 6:42 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:36 p.m.)
Indexed	1 month, 4 weeks ago (July 4, 2025, 5:46 a.m.)
Issued	24 years, 8 months ago (Jan. 2, 2001)
Published	24 years, 8 months ago (Jan. 2, 2001)
Published Online	24 years, 8 months ago (Jan. 2, 2001)
Published Print	24 years, 7 months ago (Jan. 16, 2001)

Funders 0

None

BibTeX

@article{Buetow_2001, title={High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.98.2.581}, DOI={10.1073/pnas.98.2.581}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Buetow, Kenneth H. and Edmonson, Michael and MacDonald, Richard and Clifford, Robert and Yip, Ping and Kelley, Jenny and Little, Daniel P. and Strausberg, Robert and Koester, Hubert and Cantor, Charles R. and Braun, Andreas}, year={2001}, month=jan, pages={581–584} }

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