DOI: 10.1073/pnas.97.7.3538. The RheA repressor is the thermosensor of the HSP18 heat shock response in <i>Streptomyces albus</i>

The RheA repressor is the thermosensor of the HSP18 heat shock response in Streptomyces albus

10.1073/pnas.97.7.3538

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Microorganisms have mechanisms to sense their environment and rapidly adapt to survive changes in conditions. In Streptomyces albus , various transcriptional repressors mediate the induction of heat shock genes. The RheA repressor regulates the synthesis of HSP18, a small heat shock protein, which plays a role in thermotolerance. The RheA protein was purified to determine how it responds rapidly to temperature. Gel retardation assays and footprinting experiments identified the specific target of RheA as an inverted repeat (TGTCATC 5N GATGACA) located in P hsp18 , P rheA which is the common promoter region of the divergon. Gel retardation assays detected RheA-complexes formed with the hsp18-rheA promoters. The complexes did not form at higher temperature. In vitro transcription experiments showed that RheA is an autoregulatory protein and that its activity is inhibited by high temperature. The temperature-induced derepression by RheA is reversible. Dichroism circular spectroscopy revealed a reversible change of RheA conformation in relation with the temperature that could represent a transition between an active and an inactive form. Our experiments demonstrate that RheA acts as a cellular thermometer in hsp18 regulation.

Bibliography

Servant, P., Grandvalet, C., & Mazodier, P. (2000). The RheA repressor is the thermosensor of the HSP18 heat shock response in Streptomyces albus. Proceedings of the National Academy of Sciences, 97(7), 3538â3543.

Authors 3

Pascale Servant (first)
Cosette Grandvalet (additional)
Philippe Mazodier (additional)

References 44 Referenced 48

10.1146/annurev.bi.62.070193.002025
10.1146/annurev.cb.09.110193.003125
10.1111/j.1365-2958.1993.tb01727.x
10.1146/annurev.mi.47.100193.001541
10.1046/j.1365-2958.1999.01152.x
10.1046/j.1365-2958.1996.396932.x
10.1128/jb.178.4.1088-1093.1996
10.1128/jb.177.22.6462-6468.1995
10.1046/j.1365-2958.1998.00977.x
10.1046/j.1365-2958.1996.438968.x
10.1128/jb.178.7.1919-1927.1996
10.1021/bi00163a042
10.1126/science.8421783
10.1016/S1097-2765(00)80118-5
10.1046/j.1365-2958.1998.01049.x
10.1073/pnas.90.23.11019
10.1002/j.1460-2075.1996.tb00393.x
10.1002/j.1460-2075.1995.tb07253.x
10.1073/pnas.92.8.3516
10.1093/emboj/16.15.4579
10.1101/gad.13.6.655
10.1128/iai.59.3.745-749.1991
10.1038/344682a0
10.1128/jb.175.19.6142-6149.1993
10.1046/j.1365-2958.1998.00872.x
10.1016/S0092-8674(00)80313-X
10.1128/JB.180.19.5129-5134.1998
10.1046/j.1365-2958.1997.1811563.x
10.1128/jb.179.19.5999-6004.1997
10.1128/jb.178.24.7031-7036.1996
10.1099/00221287-145-9-2385
T J Gibson Ph.D. thesis (Cambridge Univ., Cambridge, U.K., 1984). / Ph.D. thesis by Gibson T J (1984)
10.1016/0022-2836(86)90385-2
10.1073/pnas.69.8.2110
Sambrook J. Frisch E. F. & Maniatis T. (1989) (Cold Spring Harbor Lab. Press Plainview NY) 2nd Ed.
10.1038/227680a0
10.1016/0003-2697(76)90527-3
10.1016/0378-1119(82)90185-8
10.1002/j.1460-2075.1993.tb05987.x
10.1016/0092-8674(88)90472-2
10.1128/jb.177.11.2998-3003.1995
10.1128/jb.173.22.7382-7386.1991
10.1046/j.1365-2958.1999.01193.x
10.1126/science.252.5009.1162

Dates

Type	When
Created	13 years, 1 month ago (July 25, 2012, 1:37 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:25 p.m.)
Indexed	1 month ago (Aug. 2, 2025, 12:09 a.m.)
Issued	25 years, 5 months ago (March 14, 2000)
Published	25 years, 5 months ago (March 14, 2000)
Published Online	25 years, 5 months ago (March 14, 2000)
Published Print	25 years, 5 months ago (March 28, 2000)

Funders 0

None

BibTeX

@article{Servant_2000, title={The RheA repressor is the thermosensor of the HSP18 heat shock response in Streptomyces albus}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.7.3538}, DOI={10.1073/pnas.97.7.3538}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Servant, Pascale and Grandvalet, Cosette and Mazodier, Philippe}, year={2000}, month=mar, pages={3538–3543} }

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