DOI: 10.1073/pnas.97.4.1473. Self-assembly of large, ordered lamellae from non-bilayer lipids and integral membrane proteins<i>in vitro</i>

Self-assembly of large, ordered lamellae from non-bilayer lipids and integral membrane proteinsin vitro

10.1073/pnas.97.4.1473

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In many biological membranes, the major lipids are “non-bilayer lipids,” which in purified form cannot be arranged in a lamellar structure. The structural and functional roles of these lipids are poorly understood. This work demonstrates that thein vitroassociation of the two main components of a membrane, the non-bilayer lipid monogalactosyldiacylglycerol (MGDG) and the chlorophyll-a/b light-harvesting antenna protein of photosystem II (LHCII) of pea thylakoids, leads to the formation of large, ordered lamellar structures: (i) thin-section electron microscopy and circular dichroism spectroscopy reveal that the addition of MGDG induces the transformation of isolated, disordered macroaggregates of LHCII into stacked lamellar aggregates with a long-range chiral order of the complexes; (ii) small-angle x-ray scattering discloses that LHCII perturbs the structure of the pure lipid and destroys the inverted hexagonal phase; and (iii) an analysis of electron micrographs of negatively stained 2D crystals indicates that in MGDG-LHCII the complexes are found in an ordered macroarray. It is proposed that, by limiting the space available for MGDG in the macroaggregate, LHCII inhibits formation of the inverted hexagonal phase of lipids; in thylakoids, a spatial limitation is likely to be imposed by the high concentration of membrane-associated proteins.

Bibliography

Simidjiev, I., Stoylova, S., Amenitsch, H., JÃ¡vorfi, T., MustÃ¡rdy, L., Laggner, P., Holzenburg, A., & Garab, G. (2000). Self-assembly of large, ordered lamellae from non-bilayer lipids and integral membrane proteinsin vitro. Proceedings of the National Academy of Sciences, 97(4), 1473â1476.

Authors 8

Ilian Simidjiev (first)
Svetla Stoylova (additional)
Heinz Amenitsch (additional)
Tamás Jávorfi (additional)
László Mustárdy (additional)
Peter Laggner (additional)
Andreas Holzenburg (additional)
Győző Garab (additional)

References 34 Referenced 137

10.1038/386129a0
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10.1016/S0005-2736(97)00047-3
N Murata, P A Siegenthaler Lipids in Photosynthesis: Structure, Function and Genetics, eds P A Siegenthaler, N Murata (Kluwer, Dordrecht, the Netherlands), pp. 1–20 (1997). / Lipids in Photosynthesis: Structure, Function and Genetics by Murata N (1997)
10.1016/0968-0004(83)90366-3
10.1016/0005-2760(81)90167-3
W P Williams Lipids in Photosynthesis: Structure, Function and Genetics, eds N Murata, P A Siegenthaler (Kluwer, Dordrecht, the Netherlands), pp. 103–118 (1997). / Lipids in Photosynthesis: Structure, Function and Genetics by Williams W P (1997)
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10.1083/jcb.84.1.40
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R Douce, J Joyard Oxygenic Photosynthesis: The Light Reactions, eds D R Ort, C F Yocum (Kluwer, Dordrecht, the Netherlands), pp. 69–101 (1996). (10.1007/0-306-48127-8_6) / Oxygenic Photosynthesis: The Light Reactions by Douce R (1996)
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G Garab Biophysical Techniques in Photosynthesis, eds J Amesz, A J Hoff (Kluwer, Dordrecht, the Netherlands), pp. 11–40 (1996). / Biophysical Techniques in Photosynthesis by Garab G (1996)
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T Jávorfi, H Amenitsch, P Laggner, Z Cseh, L Mustárdy, S Borbély, L Rosta, G Garab Photosynthesis: Mechanisms and Effects, ed G Garab (Kluwer, Dordrecht, the Netherlands), pp. 349–352 (1998). (10.1007/978-94-011-3953-3_82) / Photosynthesis: Mechanisms and Effects by Jávorfi T (1998)
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10.1073/pnas.96.14.8277

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:20 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:56 a.m.)
Issued	25 years, 6 months ago (Feb. 15, 2000)
Published	25 years, 6 months ago (Feb. 15, 2000)
Published Online	25 years, 6 months ago (Feb. 15, 2000)
Published Print	25 years, 6 months ago (Feb. 15, 2000)

Funders 0

None

BibTeX

@article{Simidjiev_2000, title={Self-assembly of large, ordered lamellae from non-bilayer lipids and integral membrane proteinsin vitro}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.4.1473}, DOI={10.1073/pnas.97.4.1473}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Simidjiev, Ilian and Stoylova, Svetla and Amenitsch, Heinz and Jávorfi, Tamás and Mustárdy, László and Laggner, Peter and Holzenburg, Andreas and Garab, Győző}, year={2000}, month=feb, pages={1473–1476} }

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