DOI: 10.1073/pnas.97.26.14813. Characterization of Mbb1, a nucleus-encoded tetratricopeptide-like repeat protein required for expression of the chloroplast psbB / psbT / psbH gene cluster in Chlamydomonas reinhardtii

Characterization of Mbb1, a nucleus-encoded tetratricopeptide-like repeat protein required for expression of the chloroplast psbB / psbT / psbH gene cluster in Chlamydomonas reinhardtii

10.1073/pnas.97.26.14813

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this process, we have cloned the nuclear gene encoding the Mbb1 factor by genomic rescue of a mutant specifically deficient in the accumulation of the mRNAs of the psbB / psbT / psbH chloroplast transcription unit. Mbb1 is a soluble protein in the stromal phase of the chloroplast. It consists of 662 amino acids with a putative chloroplast-transit peptide at its N-terminal end. A striking feature is the presence of 10 tandemly arranged tetratricopeptide-like repeats that account for half of the protein sequence and are thought to be involved in protein–protein interactions. The Mbb1 protein seems to have a homologue in higher plants and is part of a 300-kDa complex that is associated with RNA. This complex is most likely involved in psbB mRNA processing, stability, and/or translation.

Bibliography

Vaistij, F. E., Boudreau, E., Lemaire, S. D., Goldschmidt-Clermont, M., & Rochaix, J.-D. (2000). Characterization of Mbb1, a nucleus-encoded tetratricopeptide-like repeat protein required for expression of the chloroplast psbB / psbT / psbH gene cluster in Chlamydomonas reinhardtii. Proceedings of the National Academy of Sciences, 97(26), 14813â14818.

Authors 5

Fabián E. Vaistij (first)
Eric Boudreau (additional)
Stéphane D. Lemaire (additional)
Michel Goldschmidt-Clermont (additional)
Jean-David Rochaix (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 1:13 a.m.)
Indexed	2 days, 16 hours ago (Aug. 29, 2025, 6:46 a.m.)
Issued	24 years, 8 months ago (Dec. 19, 2000)
Published	24 years, 8 months ago (Dec. 19, 2000)
Published Online	24 years, 8 months ago (Dec. 19, 2000)
Published Print	24 years, 8 months ago (Dec. 19, 2000)

Funders 0

None

BibTeX

@article{Vaistij_2000, title={Characterization of Mbb1, a nucleus-encoded tetratricopeptide-like repeat protein required for expression of the chloroplast psbB / psbT / psbH gene cluster in Chlamydomonas reinhardtii}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.26.14813}, DOI={10.1073/pnas.97.26.14813}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Vaistij, Fabián E. and Boudreau, Eric and Lemaire, Stéphane D. and Goldschmidt-Clermont, Michel and Rochaix, Jean-David}, year={2000}, month=dec, pages={14813–14818} }

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