DOI: 10.1073/pnas.97.26.14789. Structure–function analysis of the tobacco mosaic virus resistance gene <i>N</i>

Structure–function analysis of the tobacco mosaic virus resistance gene N

10.1073/pnas.97.26.14789

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The tobacco N gene is a member of the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR-NBS-LRR) class of plant resistance ( R ) genes and confers resistance to tobacco mosaic virus (TMV). We investigated the importance of specific domains of N in inducing TMV resistance, by examining various N deletion and point mutations that introduce single amino acid substitution mutants in vivo . Our deletion analysis suggests that the TIR, NBS, and LRR domains play an indispensable role in the induction of resistance responses against TMV. We show that amino acids conserved among the Toll/IL-1R/plant R gene TIR domain and NBS-containing proteins play a critical role in N -mediated TMV resistance. Some loss-of-function N alleles such as the TIR deletion and point mutations in the NBS (G216A/E/V/R, G218R, G219D, K222E/N, and T223A/N) interfere with the wild-type N function and behave like dominant negative mutations. These F 1 plants mount a hypersensitive response (HR) that is indistinguishable from that of the wild-type N plants, yet TMV was able to move systemically, causing a systemic hypersensitive response (SHR). Many amino acid substitutions in the TIR, NBS, and LRR domains of N lead to a partial loss-of-function phenotype. These mutant plants mount delayed HR compared with the wild-type N plants and fail to contain the virus to the infection site. In addition, some partial loss-of-function alleles (W82S/A, W141S/A, G218V/S, and G219V) interfere with the wild-type N function, leading to SHR. The partial loss-of-function and dominant negative mutant alleles described in this report will be useful in furthering our understanding of the TIR-NBS-LRR class of R genes.

Bibliography

Dinesh-Kumar, S. P., Tham, W.-H., & Baker, B. J. (2000). Structureâfunction analysis of the tobacco mosaic virus resistance gene N. Proceedings of the National Academy of Sciences, 97(26), 14789â14794.

Authors 3

S. P. Dinesh-Kumar (first)
Wai-Hong Tham (additional)
Barbara J. Baker (additional)

References 60 Referenced 219

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:36 p.m.)
Indexed	2 months ago (June 28, 2025, 12:01 p.m.)
Issued	24 years, 8 months ago (Dec. 19, 2000)
Published	24 years, 8 months ago (Dec. 19, 2000)
Published Online	24 years, 8 months ago (Dec. 19, 2000)
Published Print	24 years, 8 months ago (Dec. 19, 2000)

Funders 0

None

BibTeX

@article{Dinesh_Kumar_2000, title={Structure–function analysis of the tobacco mosaic virus resistance gene N}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.26.14789}, DOI={10.1073/pnas.97.26.14789}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Dinesh-Kumar, S. P. and Tham, Wai-Hong and Baker, Barbara J.}, year={2000}, month=dec, pages={14789–14794} }

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