DOI: 10.1073/pnas.97.21.11303. Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesis

Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesis

10.1073/pnas.97.21.11303

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

PCAF plays a role in transcriptional activation, cell-cycle arrest, and cell differentiation in cultured cells. PCAF contributes to transcriptional activation by acetylating chromatin and transcription factors through its intrinsic histone acetylase activity. In this report, we present evidence for the in vivo function of PCAF and the closely related PCAF-B/GCN5. Mice lacking PCAF are developmentally normal without a distinct phenotype. In PCAF null-zygous mice, protein levels of PCAF-B/GCN5 are drastically elevated in lung and liver, where PCAF is abundantly expressed in wild-type mice, suggesting that PCAF-B/GCN5 functionally compensates for PCAF. In contrast, animals lacking PCAF-B/GCN5 die between days 9.5 and 11.5 of gestation. Normally, PCAF-B / GCN5 mRNA is expressed at high levels already by day 8, whereas PCAF mRNA is first detected on day 12.5, which may explain, in part, the distinct knockout phenotypes. These results provide evidence that PCAF and PCAF-B/GCN5 play distinct but functionally overlapping roles in embryogenesis.

Bibliography

Yamauchi, T., Yamauchi, J., Kuwata, T., Tamura, T., Yamashita, T., Bae, N., Westphal, H., Ozato, K., & Nakatani, Y. (2000). Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesis. Proceedings of the National Academy of Sciences, 97(21), 11303â11306.

Authors 9

Teruo Yamauchi (first)
Jun Yamauchi (additional)
Takeshi Kuwata (additional)
Tomohiko Tamura (additional)
Tsuyoshi Yamashita (additional)
Nancy Bae (additional)
Heiner Westphal (additional)
Keiko Ozato (additional)
Yoshihiro Nakatani (additional)

References 20 Referenced 191

10.1038/382319a0
10.1002/(SICI)1521-1878(199808)20:8<615::AID-BIES4>3.0.CO;2-H
10.1101/gad.12.5.599
10.1146/annurev.biochem.67.1.545
R L Schiltz, Y Nakatani Biochim Biophys Acta 1470, M37–M53 (2000). / Biochim Biophys Acta by Schiltz R L (2000)
10.1101/gad.12.18.2831
10.1128/MCB.19.2.1202
10.1016/S1097-2765(00)80383-4
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10.1073/pnas.94.19.10215
10.1016/S0092-8674(00)81165-4

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:52 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 5, 2025, 8:57 a.m.)
Issued	24 years, 10 months ago (Oct. 10, 2000)
Published	24 years, 10 months ago (Oct. 10, 2000)
Published Online	24 years, 10 months ago (Oct. 10, 2000)
Published Print	24 years, 10 months ago (Oct. 10, 2000)

Funders 0

None

BibTeX

@article{Yamauchi_2000, title={Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesis}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.21.11303}, DOI={10.1073/pnas.97.21.11303}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yamauchi, Teruo and Yamauchi, Jun and Kuwata, Takeshi and Tamura, Tomohiko and Yamashita, Tsuyoshi and Bae, Nancy and Westphal, Heiner and Ozato, Keiko and Nakatani, Yoshihiro}, year={2000}, month=oct, pages={11303–11306} }

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  "abstract": "<jats:p>\n            PCAF plays a role in transcriptional activation, cell-cycle arrest,\n and cell differentiation in cultured cells. PCAF contributes to\n transcriptional activation by acetylating chromatin and transcription\n factors through its intrinsic histone acetylase activity. In this\n report, we present evidence for the\n            <jats:italic>in vivo</jats:italic>\n            function of\n PCAF and the closely related PCAF-B/GCN5. Mice lacking PCAF are\n developmentally normal without a distinct phenotype. In\n            <jats:italic>PCAF</jats:italic>\n            null-zygous mice, protein levels of PCAF-B/GCN5\n are drastically elevated in lung and liver, where PCAF is abundantly\n expressed in wild-type mice, suggesting that PCAF-B/GCN5 functionally\n compensates for PCAF. In contrast, animals lacking PCAF-B/GCN5 die\n between days 9.5 and 11.5 of gestation. Normally,\n            <jats:italic>PCAF-B</jats:italic>\n            /\n            <jats:italic>GCN5</jats:italic>\n            mRNA is expressed at high\n levels already by day 8, whereas\n            <jats:italic>PCAF</jats:italic>\n            mRNA is first\n detected on day 12.5, which may explain, in part, the distinct knockout\n phenotypes. These results provide evidence that PCAF and PCAF-B/GCN5\n play distinct but functionally overlapping roles in embryogenesis.\n          </jats:p>",
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