DOI: 10.1073/pnas.97.2.859. Genetic analysis of a bacterial genetic exchange element: The gene transfer agent of <i>Rhodobacter capsulatus</i>

Genetic analysis of a bacterial genetic exchange element: The gene transfer agent of Rhodobacter capsulatus

10.1073/pnas.97.2.859

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

An unusual system of genetic exchange exists in the purple nonsulfur bacterium Rhodobacter capsulatus . DNA transmission is mediated by a small bacteriophage-like particle called the gene transfer agent (GTA) that transfers random 4.5-kb segments of the producing cell's genome to recipient cells, where allelic replacement occurs. This paper presents the results of gene cloning, analysis, and mutagenesis experiments that show that GTA resembles a defective prophage related to bacteriophages from diverse genera of bacteria, which has been adopted by R. capsulatus for genetic exchange. A pair of cellular proteins, CckA and CtrA, appear to constitute part of a sensor kinase/response regulator signaling pathway that is required for expression of GTA structural genes. This signaling pathway controls growth-phase-dependent regulation of GTA gene messages, yielding maximal gene expression in the stationary phase. We suggest that GTA is an ancient prophage remnant that has evolved in concert with the bacterial genome, resulting in a genetic exchange process controlled by the bacterial cell.

Bibliography

Lang, A. S., & Beatty, J. T. (2000). Genetic analysis of a bacterial genetic exchange element: The gene transfer agent of Rhodobacter capsulatus. Proceedings of the National Academy of Sciences, 97(2), 859â864.

Authors 2

Andrew S. Lang (first)
J. T. Beatty (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 12:52 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 6, 2025, 8:59 a.m.)
Issued	25 years, 7 months ago (Jan. 18, 2000)
Published	25 years, 7 months ago (Jan. 18, 2000)
Published Online	25 years, 7 months ago (Jan. 18, 2000)
Published Print	25 years, 7 months ago (Jan. 18, 2000)

Funders 0

None

BibTeX

@article{Lang_2000, title={Genetic analysis of a bacterial genetic exchange element: The gene transfer agent of Rhodobacter capsulatus}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.2.859}, DOI={10.1073/pnas.97.2.859}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lang, Andrew S. and Beatty, J. T.}, year={2000}, month=jan, pages={859–864} }

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