DOI: 10.1073/pnas.97.19.10395. Molecular basis for CD40 signaling mediated by TRAF3

Molecular basis for CD40 signaling mediated by TRAF3

10.1073/pnas.97.19.10395

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Tumor necrosis factor receptors (TNFR) are single transmembrane-spanning glycoproteins that bind cytokines and trigger multiple signal transduction pathways. Many of these TNFRs rely on interactions with TRAF proteins that bind to the intracellular domain of the receptors. CD40 is a member of the TNFR family that binds to several different TRAF proteins. We have determined the crystal structure of a 20-residue fragment from the cytoplasmic domain of CD40 in complex with the TRAF domain of TRAF3. The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. Residues shown by mutagenesis and deletion analysis to be critical for TRAF3 binding are involved either in direct contact with TRAF3 or in intramolecular interactions that stabilize the hairpin. Comparison of the interactions of CD40 with TRAF3 vs. TRAF2 suggests that CD40 may assume different conformations when bound to different TRAF family members. Thismolecular adaptationmay influence binding affinity and specific cellular triggers.

Bibliography

Ni, C.-Z., Welsh, K., Leo, E., Chiou, C., Wu, H., Reed, J. C., & Ely, K. R. (2000). Molecular basis for CD40 signaling mediated by TRAF3. Proceedings of the National Academy of Sciences, 97(19), 10395â10399.

Authors 7

Chao-Zhou Ni (first)
Kate Welsh (additional)
Eugen Leo (additional)
Chu-kuan Chiou (additional)
Hao Wu (additional)
John C. Reed (additional)
Kathryn R. Ely (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:34 a.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 8:32 a.m.)
Issued	24 years, 11 months ago (Sept. 12, 2000)
Published	24 years, 11 months ago (Sept. 12, 2000)
Published Online	24 years, 11 months ago (Sept. 12, 2000)
Published Print	24 years, 11 months ago (Sept. 12, 2000)

Funders 0

None

BibTeX

@article{Ni_2000, title={Molecular basis for CD40 signaling mediated by TRAF3}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.19.10395}, DOI={10.1073/pnas.97.19.10395}, number={19}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ni, Chao-Zhou and Welsh, Kate and Leo, Eugen and Chiou, Chu-kuan and Wu, Hao and Reed, John C. and Ely, Kathryn R.}, year={2000}, month=sep, pages={10395–10399} }

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