DOI: 10.1073/pnas.97.17.9482. Myosin-V stepping kinetics: A molecular model for processivity

Myosin-V stepping kinetics: A molecular model for processivity

10.1073/pnas.97.17.9482

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete ≈36-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.

Bibliography

Rief, M., Rock, R. S., Mehta, A. D., Mooseker, M. S., Cheney, R. E., & Spudich, J. A. (2000). Myosin-V stepping kinetics: A molecular model for processivity. Proceedings of the National Academy of Sciences, 97(17), 9482â9486.

Authors 6

Matthias Rief (first)
Ronald S. Rock (additional)
Amit D. Mehta (additional)
Mark S. Mooseker (additional)
Richard E. Cheney (additional)
James A. Spudich (additional)

References 28 Referenced 362

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:19 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 7:29 a.m.)
Issued	25 years ago (Aug. 15, 2000)
Published	25 years ago (Aug. 15, 2000)
Published Online	25 years ago (Aug. 15, 2000)
Published Print	25 years ago (Aug. 15, 2000)

Funders 0

None

BibTeX

@article{Rief_2000, title={Myosin-V stepping kinetics: A molecular model for processivity}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.17.9482}, DOI={10.1073/pnas.97.17.9482}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rief, Matthias and Rock, Ronald S. and Mehta, Amit D. and Mooseker, Mark S. and Cheney, Richard E. and Spudich, James A.}, year={2000}, month=aug, pages={9482–9486} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        8
      ]
    ],
    "date-time": "2025-07-08T11:29:52Z",
    "timestamp": 1751974192349
  },
  "reference-count": 28,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "17",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        8,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete \u224836-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.</jats:p>",
  "DOI": "10.1073/pnas.97.17.9482",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:31:39Z",
    "timestamp": 1027693899000
  },
  "page": "9482-9486",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 362,
  "title": "Myosin-V stepping kinetics: A molecular model for processivity",
  "prefix": "10.1073",
  "volume": "97",
  "author": [
    {
      "given": "Matthias",
      "family": "Rief",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599"
        }
      ]
    },
    {
      "given": "Ronald S.",
      "family": "Rock",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599"
        }
      ]
    },
    {
      "given": "Amit D.",
      "family": "Mehta",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599"
        }
      ]
    },
    {
      "given": "Mark S.",
      "family": "Mooseker",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599"
        }
      ]
    },
    {
      "given": "Richard E.",
      "family": "Cheney",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599"
        }
      ]
    },
    {
      "given": "James A.",
      "family": "Spudich",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2000,
        8,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_2_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(05)80080-7"
    },
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-4889(00)00007-0"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/39247"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/23072"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ph.58.030196.003503"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.39.27448"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.24.13726"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(98)98003-X"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/368113a0"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(96)79746-1"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0091-679X(08)60402-1"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/348348a0"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90060-4"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/365721a0"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.282.5390.902"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(98)98040-5"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/22146"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.5.1913"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/SQB.1995.060.01.085"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(97)78231-6"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/41118"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.25.11782"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/41111"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.6.4329"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/16403"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi9722406"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.26.14332"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81139-3"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.97.17.9482",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T21:19:19Z",
    "timestamp": 1649884759000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.97.17.9482"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        8,
        15
      ]
    ]
  },
  "references-count": 28,
  "journal-issue": {
    "issue": "17",
    "published-print": {
      "date-parts": [
        [
          2000,
          8,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.97.17.9482"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.97.17.9482",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2000,
        8,
        15
      ]
    ]
  },
  "assertion": [
    {
      "value": "2000-06-07",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2000-08-15",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}