DOI: 10.1073/pnas.97.17.9419. Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarrays

Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarrays

10.1073/pnas.97.17.9419

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We used DNA microarrays of the Escherichia coli genome to trace the progression of chromosomal replication forks in synchronized cells. We found that both DNA gyrase and topoisomerase IV (topo IV) promote replication fork progression. When both enzymes were inhibited, the replication fork stopped rapidly. The elongation rate with topo IV alone was 1/3 of normal. Genetic data confirmed and extended these results. Inactivation of gyrase alone caused a slow stop of replication. Topo IV activity was sufficient to prevent accumulation of (+) supercoils in plasmid DNA in vivo , suggesting that topo IV can promote replication by removing (+) supercoils in front of the chromosomal fork.

Bibliography

Khodursky, A. B., Peter, B. J., Schmid, M. B., DeRisi, J., Botstein, D., Brown, P. O., & Cozzarelli, N. R. (2000). Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarrays. Proceedings of the National Academy of Sciences, 97(17), 9419â9424.

Authors 7

Arkady B. Khodursky (first)
Brian J. Peter (additional)
Molly B. Schmid (additional)
Joseph DeRisi (additional)
David Botstein (additional)
Patrick O. Brown (additional)
Nicholas R. Cozzarelli (additional)

References 37 Referenced 168

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10.1111/j.1365-2958.1994.tb01297.x
T Maniatis, E F Fritsch, J Sambrook Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY) 1, 1.12 (1982). / Molecular Cloning: A Laboratory Manual by Maniatis T (1982)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:21 p.m.)
Indexed	1 year, 1 month ago (Aug. 6, 2024, 12:33 a.m.)
Issued	25 years ago (Aug. 15, 2000)
Published	25 years ago (Aug. 15, 2000)
Published Online	25 years ago (Aug. 15, 2000)
Published Print	25 years ago (Aug. 15, 2000)

Funders 0

None

BibTeX

@article{Khodursky_2000, title={Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarrays}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.17.9419}, DOI={10.1073/pnas.97.17.9419}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Khodursky, Arkady B. and Peter, Brian J. and Schmid, Molly B. and DeRisi, Joseph and Botstein, David and Brown, Patrick O. and Cozzarelli, Nicholas R.}, year={2000}, month=aug, pages={9419–9424} }

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