DOI: 10.1073/pnas.97.14.7784. <i>Escherichia coli</i> CspA-family RNA chaperones are transcription antiterminators

Escherichia coli CspA-family RNA chaperones are transcription antiterminators

10.1073/pnas.97.14.7784

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

CspA, the major cold-shock protein of Escherichia coli , is an RNA chaperone, which is thought to facilitate translation at low temperature by destabilizing mRNA structures. Here we demonstrate that CspA, as well as homologous RNA chaperones CspE and CspC, are transcription antiterminators. In vitro , the addition of physiological concentrations of recombinant CspA, CspE, or CspC decreased transcription termination at several intrinsic terminators and also decreased transcription pausing. In vivo , overexpression of cloned CspC and CspE at 37°C was sufficient to induce transcription of the metY - rpsO operon genes nusA , infB , rbfA , and pnp located downstream of multiple transcription terminators. Similar induction of downstream metY - rpsO operon genes was observed at cold shock, a condition to which the cell responds by massive overproduction of CspA. The products of nusA , infB , rbfA , and pnp —NusA, IF2, RbfA, and PNP—are known to be induced at cold shock. We propose that the cold-shock induction of nusA , infB , rbfA , and pnp occurs through transcription antitermination, which is mediated by CspA and other cold shock-induced Csp proteins.

Bibliography

Bae, W., Xia, B., Inouye, M., & Severinov, K. (2000). Escherichia coli CspA-family RNA chaperones are transcription antiterminators. Proceedings of the National Academy of Sciences, 97(14), 7784â7789.

Authors 4

Weonhye Bae (first)
Bing Xia (additional)
Masayori Inouye (additional)
Konstantin Severinov (additional)

References 41 Referenced 336

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:47 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 6, 2025, 8:01 a.m.)
Issued	25 years, 1 month ago (July 5, 2000)
Published	25 years, 1 month ago (July 5, 2000)
Published Online	25 years, 1 month ago (July 5, 2000)
Published Print	25 years, 1 month ago (July 5, 2000)

Funders 0

None

BibTeX

@article{Bae_2000, title={Escherichia coli CspA-family RNA chaperones are transcription antiterminators}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.14.7784}, DOI={10.1073/pnas.97.14.7784}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bae, Weonhye and Xia, Bing and Inouye, Masayori and Severinov, Konstantin}, year={2000}, month=jul, pages={7784–7789} }

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  "abstract": "<jats:p>\n            CspA, the major cold-shock protein of\n            <jats:italic>Escherichia coli</jats:italic>\n            , is an RNA chaperone, which is thought to facilitate translation at low temperature by destabilizing mRNA structures. Here we demonstrate that CspA, as well as homologous RNA chaperones CspE and CspC, are transcription antiterminators.\n            <jats:italic>In vitro</jats:italic>\n            , the addition of physiological concentrations of recombinant CspA, CspE, or CspC decreased transcription termination at several intrinsic terminators and also decreased transcription pausing.\n            <jats:italic>In vivo</jats:italic>\n            , overexpression of cloned CspC and CspE at 37\u00b0C was sufficient to induce transcription of the\n            <jats:italic>metY</jats:italic>\n            -\n            <jats:italic>rpsO</jats:italic>\n            operon genes\n            <jats:italic>nusA</jats:italic>\n            ,\n            <jats:italic>infB</jats:italic>\n            ,\n            <jats:italic>rbfA</jats:italic>\n            , and\n            <jats:italic>pnp</jats:italic>\n            located downstream of multiple transcription terminators. Similar induction of downstream\n            <jats:italic>metY</jats:italic>\n            -\n            <jats:italic>rpsO</jats:italic>\n            operon genes was observed at cold shock, a condition to which the cell responds by massive overproduction of CspA. The products of\n            <jats:italic>nusA</jats:italic>\n            ,\n            <jats:italic>infB</jats:italic>\n            ,\n            <jats:italic>rbfA</jats:italic>\n            , and\n            <jats:italic>pnp</jats:italic>\n            \u2014NusA, IF2, RbfA, and PNP\u2014are known to be induced at cold shock. We propose that the cold-shock induction of\n            <jats:italic>nusA</jats:italic>\n            ,\n            <jats:italic>infB</jats:italic>\n            ,\n            <jats:italic>rbfA</jats:italic>\n            , and\n            <jats:italic>pnp</jats:italic>\n            occurs through transcription antitermination, which is mediated by CspA and other cold shock-induced Csp proteins.\n          </jats:p>",
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