DOI: 10.1073/pnas.97.12.6306. Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target

Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target

10.1073/pnas.97.12.6306

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Eukaryotic nuclei contain three different types of RNA polymerases (RNAPs), each consisting of 12–18 different subunits. The evolutionarily highly conserved RNAP subunit RPB5 is shared by all three enzymes and therefore represents a key structural/functional component of all eukaryotic RNAPs. Here we present the crystal structure of the RPB5 subunit from Saccharomyces cerevisiae . The bipartite structure includes a eukaryote-specific N-terminal domain and a C-terminal domain resembling the archaeal RNAP subunit H. RPB5 has been implicated in direct protein-protein contacts with transcription factor IIB, one of the components of the RNAP II basal transcriptional machinery, and gene-specific activator proteins, such as the hepatitis B virus transactivator protein X. The experimentally mapped regions of RPB5 involved in these interactions correspond to distinct and surface-exposed α-helical structures.

Bibliography

Todone, F., Weinzierl, R. O. J., Brick, P., & Onesti, S. (2000). Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target. Proceedings of the National Academy of Sciences, 97(12), 6306â6310.

Authors 4

Flavia Todone (first)
Robert O. J. Weinzierl (additional)
Peter Brick (additional)
Silvia Onesti (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 12:49 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:47 a.m.)
Issued	25 years, 2 months ago (June 6, 2000)
Published	25 years, 2 months ago (June 6, 2000)
Published Online	25 years, 2 months ago (June 6, 2000)
Published Print	25 years, 2 months ago (June 6, 2000)

Funders 0

None

BibTeX

@article{Todone_2000, title={Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.12.6306}, DOI={10.1073/pnas.97.12.6306}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Todone, Flavia and Weinzierl, Robert O. J. and Brick, Peter and Onesti, Silvia}, year={2000}, month=jun, pages={6306–6310} }

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