DOI: 10.1073/pnas.97.10.5669. Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle

Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle

10.1073/pnas.97.10.5669

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The glyoxylate cycle is regarded as essential for postgerminative growth and seedling establishment in oilseed plants. We have identified two allelic Arabidopsis mutants, icl-1 and icl-2, which lack the glyoxylate cycle because of the absence of the key enzyme isocitrate lyase. These mutants demonstrate that the glyoxylate cycle is not essential for germination. Furthermore, photosynthesis can compensate for the absence of the glyoxylate cycle during postgerminative growth, and only when light intensity or day length is decreased does seedling establishment become compromised. The provision of exogenous sugars can overcome this growth deficiency. The icl mutants also demonstrate that the glyoxylate cycle is important for seedling survival and recovery after prolonged dark conditions that approximate growth in nature. Surprisingly, despite their inability to catalyze the net conversion of acetate to carbohydrate, mutant seedlings are able to break down storage lipids. Results suggest that lipids can be used as a source of carbon for respiration in germinating oilseeds and that products of fatty acid catabolism can pass from the peroxisome to the mitochondrion independently of the glyoxylate cycle. However, an additional anaplerotic source of carbon is required for lipid breakdown and seedling establishment. This source can be provided by the glyoxylate cycle or, in its absence, by exogenous sucrose or photosynthesis.

Bibliography

Eastmond, P. J., Germain, V., Lange, P. R., Bryce, J. H., Smith, S. M., & Graham, I. A. (2000). Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle. Proceedings of the National Academy of Sciences, 97(10), 5669â5674.

Authors 6

Peter J. Eastmond (first)
Veronique Germain (additional)
Peter R. Lange (additional)
James H. Bryce (additional)
Steven M. Smith (additional)
Ian A. Graham (additional)

References 34 Referenced 230

10.1016/S1360-1385(99)01429-6
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10.1002/j.1460-2075.1995.tb07354.x
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:17 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 9:32 a.m.)
Issued	25 years, 3 months ago (May 9, 2000)
Published	25 years, 3 months ago (May 9, 2000)
Published Online	25 years, 3 months ago (May 9, 2000)
Published Print	25 years, 3 months ago (May 9, 2000)

Funders 0

None

BibTeX

@article{Eastmond_2000, title={Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.10.5669}, DOI={10.1073/pnas.97.10.5669}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Eastmond, Peter J. and Germain, Veronique and Lange, Peter R. and Bryce, James H. and Smith, Steven M. and Graham, Ian A.}, year={2000}, month=may, pages={5669–5674} }

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