DOI: 10.1073/pnas.97.1.49. Structural interactions of fibroblast growth factor receptor with its ligands

Structural interactions of fibroblast growth factor receptor with its ligands

10.1073/pnas.97.1.49

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Fibroblast growth factors (FGFs) effect cellular responses by binding to FGF receptors (FGFRs). FGF bound to extracellular domains on the FGFR in the presence of heparin activates the cytoplasmic receptor tyrosine kinase through autophosphorylation. We have crystallized a complex between human FGF1 and a two-domain extracellular fragment of human FGFR2. The crystal structure, determined by multiwavelength anomalous diffraction analysis of the selenomethionyl protein, is a dimeric assemblage of 1:1 ligand:receptor complexes. FGF is bound at the junction between the two domains of one FGFR, and two such units are associated through receptor:receptor and secondary ligand:receptor interfaces. Sulfate ion positions appear to mark the course of heparin binding between FGF molecules through a basic region on receptor D2 domains. This dimeric assemblage provides a structural mechanism for FGF signal transduction.

Bibliography

Stauber, D. J., DiGabriele, A. D., & Hendrickson, W. A. (2000). Structural interactions of fibroblast growth factor receptor with its ligands. Proceedings of the National Academy of Sciences, 97(1), 49â54.

Authors 3

Deborah J. Stauber (first)
Anna D. DiGabriele (additional)
Wayne A. Hendrickson (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 12:52 a.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 1:51 p.m.)
Issued	25 years, 7 months ago (Jan. 4, 2000)
Published	25 years, 7 months ago (Jan. 4, 2000)
Published Online	25 years, 7 months ago (Jan. 4, 2000)
Published Print	25 years, 7 months ago (Jan. 4, 2000)

Funders 0

None

BibTeX

@article{Stauber_2000, title={Structural interactions of fibroblast growth factor receptor with its ligands}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.1.49}, DOI={10.1073/pnas.97.1.49}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Stauber, Deborah J. and DiGabriele, Anna D. and Hendrickson, Wayne A.}, year={2000}, month=jan, pages={49–54} }

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