DOI: 10.1073/pnas.97.1.377. Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation

Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation

10.1073/pnas.97.1.377

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Virally transduced genes are often silenced after integration into the host genome. Chromatin immunoprecipitation and nuclease sensitivity experiments now demonstrate that silencing of the transgene is characterized by deacetylation of histone H4 lysines and chromatin condensation. Trichostatin A treatment results in dramatic reactivation of gene expression that is preceded by histone acetylation and chromatin decondensation. Analysis of individual histone H4 lysines demonstrate that chromatin domain opening is coincident with rapid acetylation of histone H4 K5, K12, and K16 and that maintenance of the open domain is correlated with acetylation of histone H4 K8. Removal of trichostatin A results in rapid deacetylation of histone H4 K8, chromatin condensation, and transcription silencing. The results suggest that deacetylation of histone H4 lysines and coincident chromatin condensation are critically involved in the silencing of virally transduced genes.

Bibliography

Chen, W. Y., & Townes, T. M. (2000). Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation. Proceedings of the National Academy of Sciences, 97(1), 377â382.

Authors 2

Wen Yong Chen (first)
Tim M. Townes (additional)

References 25 Referenced 94

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L G Davis, W M Kuehl, J F Battey Basic Methods in Molecular Biology (Appleton & Lange, Norwalk, CT, 1994). / Basic Methods in Molecular Biology by Davis L G (1994)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:26 p.m.)
Indexed	2 months ago (June 27, 2025, 12:24 a.m.)
Issued	25 years, 7 months ago (Jan. 4, 2000)
Published	25 years, 7 months ago (Jan. 4, 2000)
Published Online	25 years, 7 months ago (Jan. 4, 2000)
Published Print	25 years, 7 months ago (Jan. 4, 2000)

Funders 0

None

BibTeX

@article{Chen_2000, title={Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.97.1.377}, DOI={10.1073/pnas.97.1.377}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chen, Wen Yong and Townes, Tim M.}, year={2000}, month=jan, pages={377–382} }

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