DOI: 10.1073/pnas.96.6.2656. The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks

The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks

10.1073/pnas.96.6.2656

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae as a protein that specifically binds to origins of DNA replication. Although ORC appears to play an essential role in the initiation of DNA replication in the cells of all eukaryotes, its interactions with DNA have not been defined in species other than budding yeast. We have characterized a Schizosaccharomyces pombe homologue of the ORC subunit, Orc4p. The homologue (Orp4p) consists of two distinct functional domains. The C-terminal domain shows strong sequence similarity to human, frog, and yeast Orc4 proteins, including conserved ATP-binding motifs. The N-terminal domain contains nine copies of the AT-hook motif found in a number of DNA-binding proteins, including the members of the HMG-I(Y) family of chromatin proteins. AT-hook motifs are known from biochemical and structural studies to mediate binding to the minor groove of AT-tracts in DNA. Orp4p is essential for viability of Sc. pombe and is expressed throughout the cell cycle. The Orp4 protein (and its isolated N-terminal domain) binds to the Sc. pombe replication origin, ars1 . The DNA binding properties of Orp4p provide a plausible explanation for the characteristic features of Sc. pombe origins of replication, which differ significantly from those of Sa. cerevisiae .

Bibliography

Chuang, R.-Y., & Kelly, T. J. (1999). The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks. Proceedings of the National Academy of Sciences, 96(6), 2656â2661.

Authors 2

Ray-Yuan Chuang (first)
Thomas J. Kelly (additional)

References 53 Referenced 177

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:51 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:31 p.m.)
Issued	26 years, 5 months ago (March 16, 1999)
Published	26 years, 5 months ago (March 16, 1999)
Published Online	26 years, 5 months ago (March 16, 1999)
Published Print	26 years, 5 months ago (March 16, 1999)

Funders 0

None

BibTeX

@article{Chuang_1999, title={The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.6.2656}, DOI={10.1073/pnas.96.6.2656}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chuang, Ray-Yuan and Kelly, Thomas J.}, year={1999}, month=mar, pages={2656–2661} }

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