DOI: 10.1073/pnas.96.6.2608. Protein folding at the air–water interface studied with x-ray reflectivity

Protein folding at the air–water interface studied with x-ray reflectivity

10.1073/pnas.96.6.2608

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We report the results of x-ray reflectivity measurements of thin films formed by different water-soluble proteins at the air–aqueous solution interface. It is demonstrated that glucose oxidase, alcohol dehydrogenase, and urease molecules denaturate at the air–aqueous solution interface to form 8- to 14-Å-thick peptide sheets. X-ray reflectivity data indicate that the spreading of a lipid monolayer at the aqueous solution surface before protein injection does not prevent proteins from unfolding. On the other hand, crosslinking of proteins results in intact enzyme layers at the subphase surface. A model that involves interaction of glucose oxidase molecules with a phospholipid monolayer is proposed. In this model, an observed decrease of the lipid electron density in the protein presence is explained in terms of “holes” in the monolayer film caused by protein molecule adsorption.

Bibliography

Gidalevitz, D., Huang, Z., & Rice, S. A. (1999). Protein folding at the airâwater interface studied with x-ray reflectivity. Proceedings of the National Academy of Sciences, 96(6), 2608â2611.

Authors 3

David Gidalevitz (first)
Zhengqing Huang (additional)
Stuart A. Rice (additional)

References 26 Referenced 76

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A Diederich, M Làsche Protein Array: An Alternate Biomolecular System, ed K Nagayama (Limerick: Japan Scientific Societies Press/Elsevier, Tokyo) 34, 205–230 (1997). / Protein Array: An Alternate Biomolecular System by Diederich A (1997)
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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:55 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 2, 2025, 1:12 a.m.)
Issued	26 years, 5 months ago (March 16, 1999)
Published	26 years, 5 months ago (March 16, 1999)
Published Online	26 years, 5 months ago (March 16, 1999)
Published Print	26 years, 5 months ago (March 16, 1999)

Funders 0

None

BibTeX

@article{Gidalevitz_1999, title={Protein folding at the air–water interface studied with x-ray reflectivity}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.6.2608}, DOI={10.1073/pnas.96.6.2608}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gidalevitz, David and Huang, Zhengqing and Rice, Stuart A.}, year={1999}, month=mar, pages={2608–2611} }

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