DOI: 10.1073/pnas.96.5.1921. Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage

Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage

10.1073/pnas.96.5.1921

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or γ irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair.

Bibliography

Raderschall, E., Golub, E. I., & Haaf, T. (1999). Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage. Proceedings of the National Academy of Sciences, 96(5), 1921â1926.

Authors 3

Elke Raderschall (first)
Efim I. Golub (additional)
Thomas Haaf (additional)

References 55 Referenced 273

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:39 p.m.)
Indexed	42 minutes ago (Aug. 25, 2025, 11:02 p.m.)
Issued	26 years, 5 months ago (March 2, 1999)
Published	26 years, 5 months ago (March 2, 1999)
Published Online	26 years, 5 months ago (March 2, 1999)
Published Print	26 years, 5 months ago (March 2, 1999)

Funders 0

None

BibTeX

@article{Raderschall_1999, title={Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.5.1921}, DOI={10.1073/pnas.96.5.1921}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Raderschall, Elke and Golub, Efim I. and Haaf, Thomas}, year={1999}, month=mar, pages={1921–1926} }

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