DOI: 10.1073/pnas.96.4.1486. Global response of <i>Saccharomyces cerevisiae</i> to an alkylating agent

Global response of Saccharomyces cerevisiae to an alkylating agent

10.1073/pnas.96.4.1486

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

DNA chip technology enables simultaneous examination of how ≈6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change. By using chips bearing oligonucleotide arrays, we show that, after exposure to the alkylating agent methyl methanesulfonate, ≈325 gene transcript levels are increased and ≈76 are decreased. Of the 21 genes that already were known to be induced by a DNA-damaging agent, 18 can be scored as inducible in this data set, and surprisingly, most of the newly identified inducible genes are induced even more strongly than these 18. We examined 42 responsive and 8 nonresponsive ORFs by conventional Northern blotting, and 48 of these 50 ORFs responded as they did by DNA chip analysis, with magnitudes displaying a correlation coefficient of 0.79. Responsive genes fall into several expected and many unexpected categories. Evidence for the induction of a program to eliminate and replace alkylated proteins is presented.

Bibliography

Jelinsky, S. A., & Samson, L. D. (1999). Global response of Saccharomyces cerevisiae to an alkylating agent. Proceedings of the National Academy of Sciences, 96(4), 1486â1491.

Authors 2

Scott A. Jelinsky (first)
Leona D. Samson (additional)

References 31 Referenced 328

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:51 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 17, 2024, 6:47 a.m.)
Issued	26 years, 6 months ago (Feb. 16, 1999)
Published	26 years, 6 months ago (Feb. 16, 1999)
Published Online	26 years, 6 months ago (Feb. 16, 1999)
Published Print	26 years, 6 months ago (Feb. 16, 1999)

Funders 0

None

BibTeX

@article{Jelinsky_1999, title={Global response of Saccharomyces cerevisiae to an alkylating agent}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.4.1486}, DOI={10.1073/pnas.96.4.1486}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Jelinsky, Scott A. and Samson, Leona D.}, year={1999}, month=feb, pages={1486–1491} }

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