DOI: 10.1073/pnas.96.26.15202. Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa

Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa

10.1073/pnas.96.26.15202

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Identification of host factors that interact with pathogens is crucial to an understanding of infectious disease, but direct screening for host mutations to aid in this task is not feasible in mammals. The nematode Caenorhabditis elegans is a genetically tractable alternative for investigating the pathogenic bacterium Pseudomonas aeruginosa . A P. aeruginosa toxin, produced at high cell density under control of the quorum-sensing regulators LasR and RhlR, rapidly and lethally paralyzes C. elegans . Loss-of-function mutations in C. elegans egl-9 , a gene required for normal egg laying, confer strong resistance to the paralysis. Thus, activation of EGL-9 or of a pathway that includes it may lead to the paralysis. The molecular identity of egl-9 was determined by transformation rescue and DNA sequencing. A mammalian homologue of EGL-9 is expressed in tissues in which exposure to P. aeruginosa could have clinical effects.

Bibliography

Darby, C., Cosma, C. L., Thomas, J. H., & Manoil, C. (1999). Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences, 96(26), 15202â15207.

Authors 4

Creg Darby (first)
Christine L. Cosma (additional)
James H. Thomas (additional)
Colin Manoil (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 12:23 a.m.)
Indexed	1 month, 2 weeks ago (July 12, 2025, 6:59 p.m.)
Issued	25 years, 8 months ago (Dec. 21, 1999)
Published	25 years, 8 months ago (Dec. 21, 1999)
Published Online	25 years, 8 months ago (Dec. 21, 1999)
Published Print	25 years, 8 months ago (Dec. 21, 1999)

Funders 0

None

BibTeX

@article{Darby_1999, title={Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.26.15202}, DOI={10.1073/pnas.96.26.15202}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Darby, Creg and Cosma, Christine L. and Thomas, James H. and Manoil, Colin}, year={1999}, month=dec, pages={15202–15207} }

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