Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells
10.1073/pnas.96.26.15044
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Yeast telomere DNA consists of a continuous, ≈330-bp tract of the heterogeneous repeat TG 1-3 with irregularly spaced, high affinity sites for the protein Rap1p. Yeast monitor, or count, the number of telomeric Rap1p C termini in a negative feedback mechanism to modulate the length of the terminal TG 1-3 repeats, and synthetic telomeres that tether Rap1p molecules adjacent to the TG 1-3 tract cause wild-type cells to maintain a shorter TG 1-3 tract. To identify trans-acting proteins required to count Rap1p molecules, these same synthetic telomeres were placed in two short telomere mutants: yku70Δ (which lack the yeast Ku70 protein) and tel1Δ (which lack the yeast ortholog of ATM). Although both mutants maintain telomeres with ≈100 bp of TG 1-3 , only yku70Δ cells maintained shorter TG 1-3 repeats in response to internal Rap1p molecules. This distinct response to internal Rap1p molecules was not caused by a variation in Rap1p site density in the TG 1-3 repeats as sequencing of tel1Δ and yku70Δ telomeres showed that both strains have only five to six Rap1p sites per 100-bp telomere. In addition, the tel1Δ short telomere phenotype was epistatic to the unregulated telomere length caused by deletion of the Rap1p C-terminal domain. Thus, the length of the TG 1-3 repeats in tel1Δ cells was independent of the number of the Rap1p C termini at the telomere. These data indicate that tel1Δ cells use an alternative mechanism to regulate telomere length that is distinct from monitoring the number of telomere binding proteins.

Bibliography

Ray, A., & Runge, K. W. (1999). Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells. Proceedings of the National Academy of Sciences, 96(26), 15044–15049.

Authors 2
  1. Alo Ray (first)
  2. Kurt W. Runge (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 12:26 a.m.)
Indexed 3 weeks, 6 days ago (Aug. 6, 2025, 8:15 a.m.)
Issued 25 years, 8 months ago (Dec. 21, 1999)
Published 25 years, 8 months ago (Dec. 21, 1999)
Published Online 25 years, 8 months ago (Dec. 21, 1999)
Published Print 25 years, 8 months ago (Dec. 21, 1999)
Funders 0

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@article{Ray_1999, title={Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.26.15044}, DOI={10.1073/pnas.96.26.15044}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ray, Alo and Runge, Kurt W.}, year={1999}, month=dec, pages={15044–15049} }