DOI: 10.1073/pnas.96.26.14854. From snapshot to movie: φ analysis of protein folding transition states taken one step further

From snapshot to movie: φ analysis of protein folding transition states taken one step further

10.1073/pnas.96.26.14854

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Kinetic anomalies in protein folding can result from changes of the kinetic ground states (D, I, and N), changes of the protein folding transition state, or both. The 102-residue protein U1A has a symmetrically curved chevron plot which seems to result mainly from changes of the transition state. At low concentrations of denaturant the transition state occurs early in the folding reaction, whereas at high denaturant concentration it moves close to the native structure. In this study we use this movement to follow continuously the formation and growth of U1A's folding nucleus by φ analysis. Although U1A's transition state structure is generally delocalized and displays a typical nucleation–condensation pattern, we can still resolve a sequence of folding events. However, these events are sufficiently coupled to start almost simultaneously throughout the transition state structure.

Bibliography

TernstrÃ¶m, T., Mayor, U., Akke, M., & Oliveberg, M. (1999). From snapshot to movie: Ï analysis of protein folding transition states taken one step further. Proceedings of the National Academy of Sciences, 96(26), 14854â14859.

Authors 4

Tomas Ternström (first)
Ugo Mayor (additional)
Mikael Akke (additional)
Mikael Oliveberg (additional)

References 51 Referenced 135

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:16 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:53 a.m.)
Issued	25 years, 8 months ago (Dec. 21, 1999)
Published	25 years, 8 months ago (Dec. 21, 1999)
Published Online	25 years, 8 months ago (Dec. 21, 1999)
Published Print	25 years, 8 months ago (Dec. 21, 1999)

Funders 0

None

BibTeX

@article{Ternstr_m_1999, title={From snapshot to movie: φ analysis of protein folding transition states taken one step further}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.26.14854}, DOI={10.1073/pnas.96.26.14854}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ternström, Tomas and Mayor, Ugo and Akke, Mikael and Oliveberg, Mikael}, year={1999}, month=dec, pages={14854–14859} }

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