DOI: 10.1073/pnas.96.26.14819. The MinC component of the division site selection system in <i>Escherichia coli</i> interacts with FtsZ to prevent polymerization

The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization

10.1073/pnas.96.26.14819

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Positioning of the Z ring at the midcell site in Escherichia coli is assured by the min system, which masks polar sites through topological regulation of MinC, an inhibitor of division. To study how MinC inhibits division, we have generated a MalE-MinC fusion that retains full biological activity. We find that MalE-MinC interacts with FtsZ and prevents polymerization without inhibiting FtsZ's GTPase activity. MalE-MinC19 has reduced ability to inhibit division, reduced affinity for FtsZ, and reduced ability to inhibit FtsZ polymerization. These results, along with MinC localization, suggest that MinC rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.

Bibliography

Hu, Z., Mukherjee, A., Pichoff, S., & Lutkenhaus, J. (1999). The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization. Proceedings of the National Academy of Sciences, 96(26), 14819â14824.

Authors 4

Zonglin Hu (first)
Amit Mukherjee (additional)
Sebastien Pichoff (additional)
Joe Lutkenhaus (additional)

References 49 Referenced 339

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:21 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 12, 2025, 6:16 p.m.)
Issued	25 years, 8 months ago (Dec. 21, 1999)
Published	25 years, 8 months ago (Dec. 21, 1999)
Published Online	25 years, 8 months ago (Dec. 21, 1999)
Published Print	25 years, 8 months ago (Dec. 21, 1999)

Funders 0

None

BibTeX

@article{Hu_1999, title={The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.26.14819}, DOI={10.1073/pnas.96.26.14819}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hu, Zonglin and Mukherjee, Amit and Pichoff, Sebastien and Lutkenhaus, Joe}, year={1999}, month=dec, pages={14819–14824} }

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