DOI: 10.1073/pnas.96.25.14517. Identification and classification of p53-regulated genes

Identification and classification of p53-regulated genes

10.1073/pnas.96.25.14517

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Sequence-specific transactivation by p53 is essential to its role as a tumor suppressor. A modified tetracycline-inducible system was established to search for transcripts that were activated soon after p53 induction. Among 9,954 unique transcripts identified by serial analysis of gene expression, 34 were increased more than 10-fold; 31 of these had not previously been known to be regulated by p53. The transcription patterns of these genes, as well as previously described p53-regulated genes, were evaluated and classified in a panel of widely studied colorectal cancer cell lines. “Class I” genes were uniformly induced by p53 in all cell lines; “class II” genes were induced in a subset of the lines; and “class III” genes were not induced in any of the lines. These genes were also distinguished by the timing of their induction, their induction by clinically relevant chemotherapeutic agents, the absolute requirement for p53 in this induction, and their inducibility by p73, a p53 homolog. The results revealed substantial heterogeneity in the transcriptional responses to p53, even in cells derived from a single epithelial cell type, and pave the way to a deeper understanding of p53 tumor suppressor action.

Bibliography

Yu, J., Zhang, L., Hwang, P. M., Rago, C., Kinzler, K. W., & Vogelstein, B. (1999). Identification and classification of p53-regulated genes. Proceedings of the National Academy of Sciences, 96(25), 14517â14522.

Authors 6

Jian Yu (first)
Lin Zhang (additional)
Paul M. Hwang (additional)
Carlo Rago (additional)
Kenneth W. Kinzler (additional)
Bert Vogelstein (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:22 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	25 years, 8 months ago (Dec. 7, 1999)
Published	25 years, 8 months ago (Dec. 7, 1999)
Published Online	25 years, 8 months ago (Dec. 7, 1999)
Published Print	25 years, 8 months ago (Dec. 7, 1999)

Funders 0

None

BibTeX

@article{Yu_1999, title={Identification and classification of p53-regulated genes}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.25.14517}, DOI={10.1073/pnas.96.25.14517}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yu, Jian and Zhang, Lin and Hwang, Paul M. and Rago, Carlo and Kinzler, Kenneth W. and Vogelstein, Bert}, year={1999}, month=dec, pages={14517–14522} }

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