Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato
10.1073/pnas.96.24.14153
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

The Bs2 resistance gene of pepper specifically recognizes and confers resistance to strains of Xanthomonas campestris pv. vesicatoria that contain the corresponding bacterial avirulence gene, avrBs2 . The involvement of avrBs2 in pathogen fitness and its prevalence in many X. campestris pathovars suggests that the Bs2 gene may be durable in the field and provide resistance when introduced into other plant species. Employing a positional cloning strategy, the Bs2 locus was isolated and the gene was identified by coexpression with avrBs2 in an Agrobacterium -mediated transient assay. A single candidate gene, predicted to encode motifs characteristic of the nucleotide binding site–leucine-rich repeat class of resistance genes, was identified. This gene specifically controlled the hypersensitive response when transiently expressed in susceptible pepper and tomato lines and in a nonhost species, Nicotiana benthamiana , and was designated as Bs2 . Functional expression of Bs2 in stable transgenic tomatoes supports its use as a source of resistance in other Solanaceous plant species.

Bibliography

Tai, T. H., Dahlbeck, D., Clark, E. T., Gajiwala, P., Pasion, R., Whalen, M. C., Stall, R. E., & Staskawicz, B. J. (1999). Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato. Proceedings of the National Academy of Sciences, 96(24), 14153–14158.

Authors 8
  1. Thomas H. Tai (first)
  2. Douglas Dahlbeck (additional)
  3. Eszter T. Clark (additional)
  4. Paresh Gajiwala (additional)
  5. Romela Pasion (additional)
  6. Maureen C. Whalen (additional)
  7. Robert E. Stall (additional)
  8. Brian J. Staskawicz (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 12:32 a.m.)
Indexed 1 week, 3 days ago (Aug. 24, 2025, 6:55 p.m.)
Issued 25 years, 9 months ago (Nov. 23, 1999)
Published 25 years, 9 months ago (Nov. 23, 1999)
Published Online 25 years, 9 months ago (Nov. 23, 1999)
Published Print 25 years, 9 months ago (Nov. 23, 1999)
Funders 0

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@article{Tai_1999, title={Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.24.14153}, DOI={10.1073/pnas.96.24.14153}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tai, Thomas H. and Dahlbeck, Douglas and Clark, Eszter T. and Gajiwala, Paresh and Pasion, Romela and Whalen, Maureen C. and Stall, Robert E. and Staskawicz, Brian J.}, year={1999}, month=nov, pages={14153–14158} }