DOI: 10.1073/pnas.96.23.13124. Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules

Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules

10.1073/pnas.96.23.13124

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cessation of transcription at specific terminator DNA sequences is used by viruses, bacteria, and eukaryotes to regulate the expression of downstream genes, but the mechanisms of transcription termination are poorly characterized. To elucidate the kinetic mechanism of termination at the intrinsic terminators of enteric bacteria, we observed, by using single-molecule light microscopy techniques, the behavior of surface-immobilized Escherichia coli RNA polymerase (RNAP) molecules in vitro . An RNAP molecule remains at a canonical intrinsic terminator for ≈64 s before releasing DNA, implying the formation of an elongation-incompetent (paused) intermediate by transcription complexes that terminate but not by those that read through the terminator. Analysis of pause lifetimes establishes a complete minimal mechanism of termination in which paused intermediate formation is both necessary and sufficient to induce release of RNAP at the terminator. The data suggest that intrinsic terminators function by a nonequilibrium process in which terminator effectiveness is determined by the relative rates of nucleotide addition and paused state entry by the transcription complex.

Bibliography

Yin, H., Artsimovitch, I., Landick, R., & Gelles, J. (1999). Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules. Proceedings of the National Academy of Sciences, 96(23), 13124â13129.

Authors 4

Hong Yin (first)
Irina Artsimovitch (additional)
Robert Landick (additional)
Jeff Gelles (additional)

References 30 Referenced 68

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:40 p.m.)
Indexed	1 year ago (Aug. 12, 2024, 9:54 a.m.)
Issued	25 years, 9 months ago (Nov. 9, 1999)
Published	25 years, 9 months ago (Nov. 9, 1999)
Published Online	25 years, 9 months ago (Nov. 9, 1999)
Published Print	25 years, 9 months ago (Nov. 9, 1999)

Funders 0

None

BibTeX

@article{Yin_1999, title={Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.23.13124}, DOI={10.1073/pnas.96.23.13124}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yin, Hong and Artsimovitch, Irina and Landick, Robert and Gelles, Jeff}, year={1999}, month=nov, pages={13124–13129} }

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