DOI: 10.1073/pnas.96.22.12784. Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: Role of epsilon protein kinase C

Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: Role of epsilon protein kinase C

10.1073/pnas.96.22.12784

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Recent epidemiological studies indicate beneficial effects of moderate ethanol consumption in ischemic heart disease. Most studies, however, focus on the effect of long-term consumption of ethanol. In this study, we determined whether brief exposure to ethanol immediately before ischemia also produces cardioprotection. In addition, because protein kinase C (PKC) has been shown to mediate protection of the heart from ischemia, we determined the role of specific PKC isozymes in ethanol-induced protection. We demonstrated that ( i ) brief exposure of isolated adult rat cardiac myocytes to 10–50 mM ethanol protected against damage induced by prolonged ischemia; ( ii ) an isozyme-selective ɛPKC inhibitor developed in our laboratory inhibited the cardioprotective effect of acute ethanol exposure; ( iii ) protection of isolated intact adult rat heart also occurred after incubation with 10 mM ethanol 20 min before global ischemia; and ( iv ) ethanol-induced cardioprotection depended on PKC activation because it was blocked by chelerythrine and GF109203X, two PKC inhibitors. Consumption of 1–2 alcoholic beverages in humans leads to blood alcohol levels of ≈10 mM. Therefore, our work demonstrates that exposure to physiologically attainable ethanol levels minutes before ischemia provides cardioprotection that is mediated by direct activation of ɛPKC in the cardiac myocytes. The potential clinical implications of our findings are discussed.

Bibliography

Chen, C.-H., Gray, M. O., & Mochly-Rosen, D. (1999). Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: Role of epsilon protein kinase C. Proceedings of the National Academy of Sciences, 96(22), 12784â12789.

Authors 3

Che-Hong Chen (first)
Mary O. Gray (additional)
Daria Mochly-Rosen (additional)

References 55 Referenced 109

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:11 p.m.)
Indexed	3 months, 1 week ago (May 23, 2025, 7:26 p.m.)
Issued	25 years, 10 months ago (Oct. 26, 1999)
Published	25 years, 10 months ago (Oct. 26, 1999)
Published Online	25 years, 10 months ago (Oct. 26, 1999)
Published Print	25 years, 10 months ago (Oct. 26, 1999)

Funders 0

None

BibTeX

@article{Chen_1999, title={Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: Role of epsilon protein kinase C}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.22.12784}, DOI={10.1073/pnas.96.22.12784}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chen, Che-Hong and Gray, Mary O. and Mochly-Rosen, Daria}, year={1999}, month=oct, pages={12784–12789} }

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