Multiple left-right asymmetry defects in Shh −/− mutant mice unveil a convergence of the Shh and retinoic acid pathways in the control of Lefty-1
10.1073/pnas.96.20.11376
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Asymmetric expression of Sonic hedgehog ( Shh ) in Hensen’s node of the chicken embryo plays a key role in the genetic cascade that controls left-right asymmetry, but its involvement in left-right specification in other vertebrates remains unclear. We show that mouse embryos lacking Shh display a variety of laterality defects, including pulmonary left isomerism, alterations of heart looping, and randomization of axial turning. Expression of the left-specific gene Lefty-1 is absent in Shh −/− embryos, suggesting that the observed laterality defects could be the result of the lack of Lefty-1 . We also demonstrate that retinoic acid (RA) controls Lefty-1 expression in a pathway downstream or parallel to Shh . Further, we provide evidence that RA controls left-right development across vertebrate species. Thus, the roles of Shh and RA in left-right specification indeed are conserved among vertebrates, and the Shh and RA pathways converge in the control of Lefty-1 .

Bibliography

Tsukui, T., Capdevila, J., Tamura, K., Ruiz-Lozano, P., Rodriguez-Esteban, C., Yonei-Tamura, S., Magallón, J., Chandraratna, R. A. S., Chien, K., Blumberg, B., Evans, R. M., & Belmonte, J. C. I. (1999). Multiple left-right asymmetry defects in Shh −/− mutant mice unveil a convergence of the Shh and retinoic acid pathways in the control of Lefty-1. Proceedings of the National Academy of Sciences, 96(20), 11376–11381.

Authors 12
  1. Tohru Tsukui (first)
  2. Javier Capdevila (additional)
  3. Koji Tamura (additional)
  4. Pilar Ruiz-Lozano (additional)
  5. Concepción Rodriguez-Esteban (additional)
  6. Sayuri Yonei-Tamura (additional)
  7. Jorge Magallón (additional)
  8. Roshantha A. S. Chandraratna (additional)
  9. Kenneth Chien (additional)
  10. Bruce Blumberg (additional)
  11. Ronald M. Evans (additional)
  12. Juan Carlos Izpisúa Belmonte (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 12:24 a.m.)
Indexed 1 day, 5 hours ago (Aug. 31, 2025, 6:26 a.m.)
Issued 25 years, 11 months ago (Sept. 28, 1999)
Published 25 years, 11 months ago (Sept. 28, 1999)
Published Online 25 years, 11 months ago (Sept. 28, 1999)
Published Print 25 years, 11 months ago (Sept. 28, 1999)
Funders 0

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@article{Tsukui_1999, title={Multiple left-right asymmetry defects in Shh −/− mutant mice unveil a convergence of the Shh and retinoic acid pathways in the control of Lefty-1}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.20.11376}, DOI={10.1073/pnas.96.20.11376}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tsukui, Tohru and Capdevila, Javier and Tamura, Koji and Ruiz-Lozano, Pilar and Rodriguez-Esteban, Concepción and Yonei-Tamura, Sayuri and Magallón, Jorge and Chandraratna, Roshantha A. S. and Chien, Kenneth and Blumberg, Bruce and Evans, Ronald M. and Belmonte, Juan Carlos Izpisúa}, year={1999}, month=sep, pages={11376–11381} }