DOI: 10.1073/pnas.96.20.11335. Mitogen-activated protein kinase kinase activity is required for the G <sub>2</sub> /M transition of the cell cycle in mammalian fibroblasts

Mitogen-activated protein kinase kinase activity is required for the G 2 /M transition of the cell cycle in mammalian fibroblasts

10.1073/pnas.96.20.11335

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The mitogen-activated protein kinase (MAPK) cascade is required for mitogenesis in somatic mammalian cells and is activated by a wide variety of oncogenic stimuli. Specific roles for this signaling module in growth were dissected by inhibiting MAPK kinase 1 (MAPKK1) activity in highly synchronized NIH 3T3 cells. In addition to the known role of this kinase in cell-cycle entry from G 0 , the level of MAPKK activity was observed to affect the kinetics of progression through both the G 1 and G 2 phases of the cell cycle in NIH 3T3 cells. Ectopic expression of dominant-negative forms of MAPKK1, which was previously shown to inhibit G 0 /G 1 progression, was found to also delay progression of cells through G 2 . In addition, treatment of cells with the specific MAPKK inhibitor PD 98059 during a synchronous S phase arrested the cells in the following G 2 phase. These data demonstrate a novel role for the MAPK cascade in progression from G 2 into mitosis in NIH 3T3 cells.

Bibliography

Wright, J. H., Munar, E., Jameson, D. R., Andreassen, P. R., Margolis, R. L., Seger, R., & Krebs, E. G. (1999). Mitogen-activated protein kinase kinase activity is required for the G 2 /M transition of the cell cycle in mammalian fibroblasts. Proceedings of the National Academy of Sciences, 96(20), 11335â11340.

Authors 7

Jocelyn H. Wright (first)
Erlynda Munar (additional)
Damon R. Jameson (additional)
Paul R. Andreassen (additional)
Robert L. Margolis (additional)
Rony Seger (additional)
Edwin G. Krebs (additional)

References 48 Referenced 143

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:45 p.m.)
Indexed	1 month ago (July 30, 2025, 10:52 a.m.)
Issued	25 years, 11 months ago (Sept. 28, 1999)
Published	25 years, 11 months ago (Sept. 28, 1999)
Published Online	25 years, 11 months ago (Sept. 28, 1999)
Published Print	25 years, 11 months ago (Sept. 28, 1999)

Funders 0

None

BibTeX

@article{Wright_1999, title={Mitogen-activated protein kinase kinase activity is required for the G 2 /M transition of the cell cycle in mammalian fibroblasts}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.20.11335}, DOI={10.1073/pnas.96.20.11335}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wright, Jocelyn H. and Munar, Erlynda and Jameson, Damon R. and Andreassen, Paul R. and Margolis, Robert L. and Seger, Rony and Krebs, Edwin G.}, year={1999}, month=sep, pages={11335–11340} }

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