DOI: 10.1073/pnas.96.2.376. The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression

The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression

10.1073/pnas.96.2.376

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The mediator complex is essential for regulated transcription in vitro . In the yeast Saccharomyces cerevisiae , mediator comprises >15 subunits and interacts with the C-terminal domain of the largest subunit of RNA polymerase II, thus forming an RNA polymerase II holoenzyme. Here we describe the molecular cloning of the MED1 cDNA encoding the 70-kDa subunit of the mediator complex. Yeast cells lacking the MED1 gene are viable but show a complex phenotype including partial defects in both repression and induction of the GAL genes. Together with results on other mediator subunits, this implies that the mediator is involved in both transcriptional activation and repression. Similar to mutations in the SRB10 and SRB11 genes encoding cyclin C and the cyclin C-dependent kinase, a disruption of the MED1 gene can partially suppress loss of the Snf1 protein kinase. We further found that a lexA-Med1 fusion protein is a strong activator in srb11 cells, which suggests a functional link between Med1 and the Srb10/11 complex. Finally, we show that the Med2 protein is lost from the mediator on purification from Med1-deficient cells, indicating a physical interaction between Med1 and Med2.

Bibliography

Balciunas, D., GÃ¤lman, C., Ronne, H., & BjÃ¶rklund, S. (1999). The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression. Proceedings of the National Academy of Sciences, 96(2), 376â381.

Authors 4

Darius Balciunas (first)
Cecilia Gälman (additional)
Hans Ronne (additional)
Stefan Björklund (additional)

References 36 Referenced 35

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:42 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:51 p.m.)
Indexed	1 year, 7 months ago (Jan. 27, 2024, 5:18 a.m.)
Issued	26 years, 7 months ago (Jan. 19, 1999)
Published	26 years, 7 months ago (Jan. 19, 1999)
Published Online	26 years, 7 months ago (Jan. 19, 1999)
Published Print	26 years, 7 months ago (Jan. 19, 1999)

Funders 0

None

BibTeX

@article{Balciunas_1999, title={The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.2.376}, DOI={10.1073/pnas.96.2.376}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Balciunas, Darius and Gälman, Cecilia and Ronne, Hans and Björklund, Stefan}, year={1999}, month=jan, pages={376–381} }

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