DOI: 10.1073/pnas.96.19.10794. Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D

Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D

10.1073/pnas.96.19.10794

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Surfactant protein D (SP-D) is an oligomeric C type lectin that promotes phagocytosis by binding to microbial surface carbohydrates. A 340-kDa glycoprotein (gp-340) has been shown to bind SP-D in the presence of calcium but does so independently of carbohydrate recognition. This protein exists both in a soluble form and in association with the membranes of alveolar macrophages. The primary structure of gp-340 has been established by molecular cloning, which yielded a 7,686-bp cDNA sequence encoding a polypeptide chain of 2,413 amino acids. The domain organization features 13 scavenger receptor cysteine-rich (SRCR) domains, each separated by an SRCR-interspersed domain, except for SRCRs 4 and 5, which are contiguous. The 13 SRCR domains are followed by two C1r/C1s Uegf Bmp1 domains separated by a 14th SRCR domain and a zona pellucida domain. gp-340 seems to be an alternative spliced form ofDMBT1. Reverse transcription–PCR analysis showed that the main sites of synthesis of gp-340 are lung, trachea, salivary gland, small intestine, and stomach. Immunohistochemistry revealed strong staining for gp-340 in alveolar and other tissue macrophages. Immunostaining of the macrophage membrane was either uniform or focal in a way that suggested capping, whereas other macrophages showed strong intracellular staining within the phagosome/phagolysosome compartments. In some macrophages, SP-D and gp-340 were located in the same cellular compartment. Immunoreactive gp-340 was also found in epithelial cells of the small intestine and in the ducts of salivary glands. The distribution of gp-340 in macrophages is compatible with a role as an opsonin receptor for SP-D.

Bibliography

Holmskov, U., Mollenhauer, J., Madsen, J., Vitved, L., GrÃ¸nlund, J., TornÃ¸e, I., Kliem, A., Reid, K. B. M., Poustka, A., & SkjÃ¸dt, K. (1999). Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D. Proceedings of the National Academy of Sciences, 96(19), 10794â10799.

Authors 10

Uffe Holmskov (first)
Jan Mollenhauer (additional)
Jens Madsen (additional)
Lars Vitved (additional)
Jørn Grønlund (additional)
Ida Tornøe (additional)
Anette Kliem (additional)
Kenneth B. M. Reid (additional)
Annemarie Poustka (additional)
Karsten Skjødt (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:30 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 12, 2025, 6:14 p.m.)
Issued	25 years, 11 months ago (Sept. 14, 1999)
Published	25 years, 11 months ago (Sept. 14, 1999)
Published Online	25 years, 11 months ago (Sept. 14, 1999)
Published Print	25 years, 11 months ago (Sept. 14, 1999)

Funders 0

None

BibTeX

@article{Holmskov_1999, title={Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.19.10794}, DOI={10.1073/pnas.96.19.10794}, number={19}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Holmskov, Uffe and Mollenhauer, Jan and Madsen, Jens and Vitved, Lars and Grønlund, Jørn and Tornøe, Ida and Kliem, Anette and Reid, Kenneth B. M. and Poustka, Annemarie and Skjødt, Karsten}, year={1999}, month=sep, pages={10794–10799} }

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