DOI: 10.1073/pnas.96.18.10016. Chip-based genotyping by mass spectrometry

Chip-based genotyping by mass spectrometry

10.1073/pnas.96.18.10016

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N -succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-μl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.

Bibliography

Tang, K., Fu, D.-J., Julien, D., Braun, A., Cantor, C. R., & KÃ¶ster, H. (1999). Chip-based genotyping by mass spectrometry. Proceedings of the National Academy of Sciences, 96(18), 10016â10020.

Authors 6

Kai Tang (first)
Dong-Jing Fu (additional)
Dominique Julien (additional)
Andreas Braun (additional)
Charles R. Cantor (additional)
Hubert Köster (additional)

References 17 Referenced 215

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:34 p.m.)
Indexed	4 weeks, 1 day ago (July 30, 2025, 10:40 a.m.)
Issued	25 years, 11 months ago (Aug. 31, 1999)
Published	25 years, 11 months ago (Aug. 31, 1999)
Published Online	25 years, 11 months ago (Aug. 31, 1999)
Published Print	25 years, 11 months ago (Aug. 31, 1999)

Funders 0

None

BibTeX

@article{Tang_1999, title={Chip-based genotyping by mass spectrometry}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.18.10016}, DOI={10.1073/pnas.96.18.10016}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tang, Kai and Fu, Dong-Jing and Julien, Dominique and Braun, Andreas and Cantor, Charles R. and Köster, Hubert}, year={1999}, month=aug, pages={10016–10020} }

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