DOI: 10.1073/pnas.96.14.8271. The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells

The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells

10.1073/pnas.96.14.8271

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Besides the cytochrome c pathway, plant mitochondria have an alternative respiratory pathway that is comprised of a single homodimeric protein, alternative oxidase (AOX). Transgenic cultured tobacco cells with altered levels of AOX were used to test the hypothesis that the alternative pathway in plant mitochondria functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Using the ROS-sensitive probe 2′,7′-dichlorofluorescein diacetate, we found that antisense suppression of AOX resulted in cells with a significantly higher level of ROS compared with wild-type cells, whereas the overexpression of AOX resulted in cells with lower ROS abundance. Laser-scanning confocal microscopy showed that the difference in ROS abundance among wild-type and AOX transgenic cells was caused by changes in mitochondrial-specific ROS formation. Mitochondrial ROS production was exacerbated by the use of antimycin A, which inhibited normal cytochrome electron transport. In addition, cells overexpressing AOX were found to have consistently lower expression of genes encoding ROS-scavenging enzymes, including the superoxide dismutase genes SodA and SodB , as well as glutathione peroxidase. Also, the abundance of mRNAs encoding salicylic acid-binding catalase and a pathogenesis-related protein were significantly higher in cells deficient in AOX. These results are evidence that AOX plays a role in lowering mitochondrial ROS formation in plant cells.

Bibliography

Maxwell, D. P., Wang, Y., & McIntosh, L. (1999). The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. Proceedings of the National Academy of Sciences, 96(14), 8271â8276.

Authors 3

Denis P. Maxwell (first)
Yong Wang (additional)
Lee McIntosh (additional)

References 48 Referenced 904

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:39 p.m.)
Indexed	9 hours, 29 minutes ago (Aug. 29, 2025, 6:46 a.m.)
Issued	26 years, 1 month ago (July 6, 1999)
Published	26 years, 1 month ago (July 6, 1999)
Published Online	26 years, 1 month ago (July 6, 1999)
Published Print	26 years, 1 month ago (July 6, 1999)

Funders 0

None

BibTeX

@article{Maxwell_1999, title={The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.14.8271}, DOI={10.1073/pnas.96.14.8271}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Maxwell, Denis P. and Wang, Yong and McIntosh, Lee}, year={1999}, month=jul, pages={8271–8276} }

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