DOI: 10.1073/pnas.96.12.6591. Accurate quantitation of protein expression and site-specific phosphorylation

Accurate quantitation of protein expression and site-specific phosphorylation

10.1073/pnas.96.12.6591

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A mass spectrometry-based method is described for simultaneous identification and quantitation of individual proteins and for determining changes in the levels of modifications at specific sites on individual proteins. Accurate quantitation is achieved through the use of whole-cell stable isotope labeling. This approach was applied to the detection of abundance differences of proteins present in wild-type versus mutant cell populations and to the identification of in vivo phosphorylation sites in the PAK-related yeast Ste20 protein kinase that depend specifically on the G1 cyclin Cln2. The present method is general and affords a quantitative description of cellular differences at the level of protein expression and modification, thus providing information that is critical to the understanding of complex biological phenomena.

Bibliography

Oda, Y., Huang, K., Cross, F. R., Cowburn, D., & Chait, B. T. (1999). Accurate quantitation of protein expression and site-specific phosphorylation. Proceedings of the National Academy of Sciences, 96(12), 6591â6596.

Authors 5

Y. Oda (first)
K. Huang (additional)
F. R. Cross (additional)
D. Cowburn (additional)
B. T. Chait (additional)

References 25 Referenced 814

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:59 p.m.)
Indexed	19 hours ago (Aug. 30, 2025, 12:45 p.m.)
Issued	26 years, 2 months ago (June 8, 1999)
Published	26 years, 2 months ago (June 8, 1999)
Published Online	26 years, 2 months ago (June 8, 1999)
Published Print	26 years, 2 months ago (June 8, 1999)

Funders 0

None

BibTeX

@article{Oda_1999, title={Accurate quantitation of protein expression and site-specific phosphorylation}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.12.6591}, DOI={10.1073/pnas.96.12.6591}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Oda, Y. and Huang, K. and Cross, F. R. and Cowburn, D. and Chait, B. T.}, year={1999}, month=jun, pages={6591–6596} }

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