DOI: 10.1073/pnas.96.11.6439. Regulation of RpoS proteolysis in <i>Escherichia coli</i> : The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS

Regulation of RpoS proteolysis in Escherichia coli : The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS

10.1073/pnas.96.11.6439

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The degradation of the RpoS (σ S ) subunit of RNA polymerase in Escherichia coli is a prime example of regulated proteolysis in prokaryotes. RpoS turnover depends on ClpXP protease, the response regulator RssB, and a hitherto uncharacterized “turnover element” within RpoS itself. Here we localize the turnover element to a small element (around the crucial amino acid lysine-173) directly downstream of the promoter-recognizing region 2.4 in RpoS. Its sequence as well as its location identify the turnover element as a unique proteolysis-promoting motif. This element is shown to be a site of interaction with RssB. Thus, RssB is functionally unique among response regulators as a direct recognition factor in ClpXP-dependent RpoS proteolysis. Binding of RssB to RpoS is stimulated by phosphorylation of the RssB receiver domain, suggesting that environmental stress affects RpoS proteolysis by modulating RssB affinity for RpoS. Initial evidence indicates that lysine-173 in RpoS, besides being essential of RpoS proteolysis, may play a role in promoter recognition. Thus the same region in RpoS is crucial for proteolysis as well as for activity as a transcription factor.

Bibliography

Becker, G., Klauck, E., & Hengge-Aronis, R. (1999). Regulation of RpoS proteolysis in Escherichia coli â¯: The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS. Proceedings of the National Academy of Sciences, 96(11), 6439â6444.

Authors 3

Gisela Becker (first)
Eberhard Klauck (additional)
Regine Hengge-Aronis (additional)

References 45 Referenced 165

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:27 p.m.)
Indexed	1 month, 1 week ago (July 23, 2025, 8:27 a.m.)
Issued	26 years, 3 months ago (May 25, 1999)
Published	26 years, 3 months ago (May 25, 1999)
Published Online	26 years, 3 months ago (May 25, 1999)
Published Print	26 years, 3 months ago (May 25, 1999)

Funders 0

None

BibTeX

@article{Becker_1999, title={Regulation of RpoS proteolysis in Escherichia coli  : The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.11.6439}, DOI={10.1073/pnas.96.11.6439}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Becker, Gisela and Klauck, Eberhard and Hengge-Aronis, Regine}, year={1999}, month=may, pages={6439–6444} }

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