DOI: 10.1073/pnas.96.11.6417. Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation

Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation

10.1073/pnas.96.11.6417

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We show that an electric treatment in the form of high-frequency, low-voltage electric pulses can increase more than 100-fold the production and secretion of a recombinant protein from mouse skeletal muscle. Therapeutical erythopoietin (EPO) levels were achieved in mice with a single injection of as little as 1 μg of plasmid DNA, and the increase in hematocrit after EPO production was stable and long-lasting. Pharmacological regulation through a tetracycline-inducible promoter allowed regulation of serum EPO and hematocrit levels. Tissue damage after stimulation was transient. The method described thus provides a potentially safe and low-cost treatment for serum protein deficiencies.

Bibliography

Rizzuto, G., Cappelletti, M., Maione, D., Savino, R., Lazzaro, D., Costa, P., Mathiesen, I., Cortese, R., Ciliberto, G., Laufer, R., La Monica, N., & Fattori, E. (1999). Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation. Proceedings of the National Academy of Sciences, 96(11), 6417â6422.

Authors 12

Gabriella Rizzuto (first)
Manuela Cappelletti (additional)
Domenico Maione (additional)
Rocco Savino (additional)
Domenico Lazzaro (additional)
Patrizia Costa (additional)
Iacob Mathiesen (additional)
Riccardo Cortese (additional)
Gennaro Ciliberto (additional)
Ralph Laufer (additional)
Nicola La Monica (additional)
Elena Fattori (additional)

References 38 Referenced 257

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:32 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 2, 2025, 12:55 a.m.)
Issued	26 years, 3 months ago (May 25, 1999)
Published	26 years, 3 months ago (May 25, 1999)
Published Online	26 years, 3 months ago (May 25, 1999)
Published Print	26 years, 3 months ago (May 25, 1999)

Funders 0

None

BibTeX

@article{Rizzuto_1999, title={Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.11.6417}, DOI={10.1073/pnas.96.11.6417}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rizzuto, Gabriella and Cappelletti, Manuela and Maione, Domenico and Savino, Rocco and Lazzaro, Domenico and Costa, Patrizia and Mathiesen, Iacob and Cortese, Riccardo and Ciliberto, Gennaro and Laufer, Ralph and La Monica, Nicola and Fattori, Elena}, year={1999}, month=may, pages={6417–6422} }

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