Abstract
The vomeronasal organ (VNO) of terrestrial vertebrates plays a key role in the detection of pheromones, chemicals released by animals that elicit stereotyped sexual and aggressive behaviors among conspecifics. Sensory transduction in the VNO appears unrelated to that in the vertebrate olfactory and visual systems: the putative pheromone receptors of the VNO are evolutionarily independent from the odorant receptors and, in contrast to vertebrate visual and olfactory transduction, vomeronasal transduction is unlikely to be mediated by cyclic-nucleotide-gated channels. We hypothesized that sensory transduction in the VNO might instead involve an ion channel of the transient receptor potential (TRP) family, members of which mediate cyclic-nucleotide-independent sensory responses in Drosophila melanogaster and Caenorhabditis elegans and play unknown functions in mammals. We have isolated a cDNA (rTRP2) from rat VNO encoding a protein of 885 amino acids that is equally distant from vertebrate and invertebrate TRP channels (10–30% amino acid identity). rTRP2 mRNA is exclusively expressed in VNO neurons, and the protein is highly localized to VNO sensory microvilli, the proposed site of pheromone sensory transduction. The absence of Ca 2+ stores in sensory microvilli suggests that, in contrast to a proposed mechanism of activation of mammalian TRP channels, but in accord with analysis of TRP function in Drosophila phototransduction, the gating of TRP2 is independent from the depletion of internal Ca 2+ stores. Thus, TRP2 is likely to participate in vomeronasal sensory transduction, which may share additional similarities with light-induced signaling in the Drosophila eye.
References
44
Referenced
344
-
M Halpern Annu Rev Neurosci 10, 325–362 (1987).
(
10.1146/annurev.ne.10.030187.001545) / Annu Rev Neurosci by Halpern M (1987) -
D Schild, D Restrepo Physiol Rev 78, 429–466 (1998).
(
10.1152/physrev.1998.78.2.429) / Physiol Rev by Schild D (1998) -
L Buck, R Axel Cell 65, 175–187 (1991).
(
10.1016/0092-8674(91)90418-X) / Cell by Buck L (1991) -
H Zhao, L Ivic, J M Otaki, M Hashimoto, K Mikoshiba, S Firestein Science 279, 237–242 (1998).
(
10.1126/science.279.5348.237) / Science by Zhao H (1998) -
J Bradley, J Li, N Davidson, H Lester, K Zinn Proc Natl Acad Sci USA 91, 8890–8894 (1994).
(
10.1073/pnas.91.19.8890) / Proc Natl Acad Sci USA by Bradley J (1994) -
E R Liman, L B Buck Neuron 13, 611–621 (1994).
(
10.1016/0896-6273(94)90029-9) / Neuron by Liman E R (1994) -
L J Brunet, G H Gold, J Ngai Neuron 17, 681–693 (1996).
(
10.1016/S0896-6273(00)80200-7) / Neuron by Brunet L J (1996) -
L Belluscio, G H Gold, A Nemes, R Axel Neuron 20, 69–81 (1998).
(
10.1016/S0896-6273(00)80435-3) / Neuron by Belluscio L (1998) -
C Dulac, R Axel Cell 83, 195–206 (1995).
(
10.1016/0092-8674(95)90161-2) / Cell by Dulac C (1995) -
A Berghard, L B Buck, E R Liman Proc Natl Acad Sci USA 93, 2365–2369 (1996).
(
10.1073/pnas.93.6.2365) / Proc Natl Acad Sci USA by Berghard A (1996) -
Y Wu, R Trindelli, N J P Ryba Biochem Biophys Res Commun 220, 900–904 (1996).
(
10.1006/bbrc.1996.0503) / Biochem Biophys Res Commun by Wu Y (1996) -
A Berghard, L B Buck J Neurosci 16, 909–918 (1996).
(
10.1523/JNEUROSCI.16-03-00909.1996) / J Neurosci by Berghard A (1996) -
G Herrada, C Dulac Cell 90, 763–773 (1997).
(
10.1016/S0092-8674(00)80536-X) / Cell by Herrada G (1997) -
H Matsunami, L B Buck Cell 90, 775–784 (1997).
(
10.1016/S0092-8674(00)80537-1) / Cell by Matsunami H (1997) -
N J Ryba, R Tirindelli Neuron 19, 371–379 (1997).
(
10.1016/S0896-6273(00)80946-0) / Neuron by Ryba N J (1997) -
E R Liman, D P Corey J Neurosci 16, 4625–4637 (1996).
(
10.1523/JNEUROSCI.16-15-04625.1996) / J Neurosci by Liman E R (1996) -
B A Niemeyer, E Suzuki, K Scott, K Jalink, C S Zuker Cell 85, 651–659 (1996).
(
10.1016/S0092-8674(00)81232-5) / Cell by Niemeyer B A (1996) -
H A Colbert, T L Smith, C I Bargmann J Neurosci 17, 8259–8269 (1997).
(
10.1523/JNEUROSCI.17-21-08259.1997) / J Neurosci by Colbert H A (1997) -
P D Wes, J Chevesich, A Jeromin, C Rosenberg, G Stetten, G Montell Proc Natl Acad Sci USA 92, 9652–9656 (1995).
(
10.1073/pnas.92.21.9652) / Proc Natl Acad Sci USA by Wes P D (1995) -
X Zhu, M Jiang, M Peyton, G Boulay, R Hurst, E Stefani, L Birnbaumer Cell 85, 661–671 (1996).
(
10.1016/S0092-8674(00)81233-7) / Cell by Zhu X (1996) -
U Wissenbach, G Schroth, S Philipp, V Flockerzi FEBS Lett 429, 61–66 (1998).
(
10.1016/S0014-5793(98)00561-4) / FEBS Lett by Wissenbach U (1998) -
G Boulay, X Zhu, M Peyton, M Jiang, R Hurst, E Stefani, L Birnbaumer J Biol Chem 272, 29672–29680 (1997).
(
10.1074/jbc.272.47.29672) / J Biol Chem by Boulay G (1997) -
T Okada, S Shimizu, M Wakamori, A Maeda, T Kurosaki, N Takada, K Imoto, Y Mori J Biol Chem 273, 10279–10287 (1998).
(
10.1074/jbc.273.17.10279) / J Biol Chem by Okada T (1998) -
S Philipp, A Cavalie, M Freichel, U Wissenbach, S Zimmer, C Trost, A Marquart, M Murakami, V Flockerzi EMBO J 15, 6166–6171 (1996).
(
10.1002/j.1460-2075.1996.tb01004.x) / EMBO J by Philipp S (1996) -
D Adams Microsc Res Technique 23, 86–97 (1992).
(
10.1002/jemt.1070230108) / Microsc Res Technique by Adams D (1992) - O L Vaccarezz, L N Sepich, J H Tramezzani J Anat 132, 167–185 (1981). / J Anat by Vaccarezz O L (1981)
-
S E Lux, K M John, V Bennett Nature (London) 344, 36–43 (1990).
(
10.1038/344036a0) / Nature (London) by Lux S E (1990) -
M J Caterina, M A Schumacher, M Tominaga, T A Rosen, J D Levine, D Julius Nature (London) 389, 816–824 (1997).
(
10.1038/39807) / Nature (London) by Caterina M J (1997) -
C J Wisocky Neurosci Biobehav Rev 3, 301–341 (1979).
(
10.1016/0149-7634(79)90015-0) / Neurosci Biobehav Rev by Wisocky C J (1979) -
K Stern, M R McClintock Nature (London) 392, 177–179 (1998).
(
10.1038/32408) / Nature (London) by Stern K (1998) -
R Ranganathan, D M Malicki, C S Zuker Annu Rev Neurosci 18, 283–317 (1995).
(
10.1146/annurev.ne.18.030195.001435) / Annu Rev Neurosci by Ranganathan R (1995) -
M Halpern, L W S Shapiro, C Jia Brain Res 677, 157–161 (1995).
(
10.1016/0006-8993(95)00159-N) / Brain Res by Halpern M (1995) -
W J Tang, A G Gilman Science 254, 1500–1503 (1991).
(
10.1126/science.1962211) / Science by Tang W J (1991) -
P C Sternweiss Curr Opin Cell Biol 6, 198–203 (1994).
(
10.1016/0955-0674(94)90136-8) / Curr Opin Cell Biol by Sternweiss P C (1994) -
Y Luo, S Lu, P Chen, D Wang, M Halpern J Biol Chem 269, 16867–16877 (1994).
(
10.1016/S0021-9258(19)89471-1) / J Biol Chem by Luo Y (1994) -
C Kroner, H Breer, A G Singer, R J O’Connell NeuroReport 7, 2989–2992 (1996).
(
10.1097/00001756-199611250-00038) / NeuroReport by Kroner C (1996) -
K S Wekesa, R R Anholt Endocrinology 138, 3497–3504 (1997).
(
10.1210/endo.138.8.5338) / Endocrinology by Wekesa K S (1997) -
D E Clapham Neuron 16, 1069–1072 (1996).
(
10.1016/S0896-6273(00)80132-4) / Neuron by Clapham D E (1996) -
K Scott, C Zuker Curr Opin Neurobiol 8, 383–388 (1998).
(
10.1016/S0959-4388(98)80065-2) / Curr Opin Neurobiol by Scott K (1998) -
B Minke, Z Selinger Curr Opin Neurobiol 6, 459–466 (1996).
(
10.1016/S0959-4388(96)80050-X) / Curr Opin Neurobiol by Minke B (1996) -
J K Acharya, K Jalink, R Hardy, V Hartenstein, C S Zuker Neuron 18, 881–887 (1997).
(
10.1016/S0896-6273(00)80328-1) / Neuron by Acharya J K (1997) -
J D Thompson, T J Gibson, F Plewniak, F Jeanmougin, D G Higgings Nucleic Acids Res 25, 4876–4882 (1997).
(
10.1093/nar/25.24.4876) / Nucleic Acids Res by Thompson J D (1997) -
S Chyb, P Raghu, R C Hardie Nature (London) 397, 255–259 (1999).
(
10.1038/16703) / Nature (London) by Chyb S (1999) -
T Hofmann, A G Obukhov, M Schaefer, C Harteneck, T Gudermann, G Schultz Nature (London) 397, 259–263 (1999).
(
10.1038/16711) / Nature (London) by Hofmann T (1999)
Dates
| Type | When |
|---|---|
| Created | 23 years, 1 month ago (July 26, 2002, 10:39 a.m.) |
| Deposited | 3 years, 2 months ago (June 7, 2022, 12:31 a.m.) |
| Indexed | 1 month, 2 weeks ago (July 11, 2025, 9:13 p.m.) |
| Issued | 26 years, 3 months ago (May 11, 1999) |
| Published | 26 years, 3 months ago (May 11, 1999) |
| Published Online | 26 years, 3 months ago (May 11, 1999) |
| Published Print | 26 years, 3 months ago (May 11, 1999) |
@article{Liman_1999, title={TRP2: A candidate transduction channel for mammalian pheromone sensory signaling}, volume={96}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.96.10.5791}, DOI={10.1073/pnas.96.10.5791}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Liman, Emily R. and Corey, David P. and Dulac, Catherine}, year={1999}, month=may, pages={5791–5796} }