DOI: 10.1073/pnas.95.6.2950. Centrosome hypertrophy in human breast tumors: Implications for genomic stability and cell polarity

Centrosome hypertrophy in human breast tumors: Implications for genomic stability and cell polarity

10.1073/pnas.95.6.2950

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The centrosome plays an important role in maintenance of cell polarity and in progression through the cell cycle by determining the number, polarity, and organization of interphase and mitotic microtubules. By examining a set of 35 high grade human breast tumors, we show that centrosomes of adenocarcinoma cells generally display abnormal structure, aberrant protein phosphorylation, and increased microtubule nucleating capacity in comparison to centrosomes of normal breast epithelial and stromal tissues. These structural and functional centrosome defects have important implications for understanding the mechanisms by which genomic instability and loss of cell polarity develop in solid tumors.

Bibliography

Lingle, W. L., Lutz, W. H., Ingle, J. N., Maihle, N. J., & Salisbury, J. L. (1998). Centrosome hypertrophy in human breast tumors: Implications for genomic stability and cell polarity. Proceedings of the National Academy of Sciences, 95(6), 2950â2955.

Authors 5

Wilma L. Lingle (first)
Ward H. Lutz (additional)
James N. Ingle (additional)
Nita J. Maihle (additional)
Jeffrey L. Salisbury (additional)

References 47 Referenced 401

10.1146/annurev.bi.63.070194.003231
C Rieder, G Borisy Biol Cell 44, 117–132 (1982). / Biol Cell by Rieder C (1982)
D Wheatley The Centriole: A Central Enigma of Cell Biology (Elsevier Biomedical, Amsterdam, 1982). / The Centriole: A Central Enigma of Cell Biology by Wheatley D (1982)
10.1016/1047-8477(92)90011-X
10.1242/jcs.107.11.2993
E B Wilson The Cell in Development and Heredity (Macmillan, New York, 1925). / The Cell in Development and Heredity by Wilson E B (1925)
10.1083/jcb.110.4.1123
10.1083/jcb.104.2.231
10.1242/jcs.107.1.9
10.1016/0955-0674(95)80043-3
10.1016/0962-8924(95)80011-5
10.1016/0092-8674(90)90693-9
10.1016/0092-8674(91)90390-K
10.1016/0092-8674(91)90389-G
10.1083/jcb.124.1.19
10.1016/0092-8674(94)90504-5
10.1002/jcp.1041600107
10.1016/0248-4900(92)90442-4
10.1242/jcs.109.13.3089
Boveri T. (1914) The Origin of Malignant Tumors trans. (1929) (Williams & Wilkins Baltimore).
10.1007/BF00690208
10.1126/science.7871434
10.1016/S0960-9822(02)00639-5
10.1126/science.271.5256.1744
H D Soule, T M Maloney, S R Wolman, W D Peterson, R Brenz, C M McGrath, J Russo, R J Pauley, R F Jones, S C Brooks Cancer Res 50, 6075–6086 (1990). / Cancer Res by Soule H D (1990)
10.1016/S0091-679X(08)60298-8
10.1016/0303-7207(93)90225-9
10.1111/j.1399-3011.1992.tb01461.x
A T Baron, R Errabolu, J Dinusson, J L Salisbury Cilia and Flagella, eds W Dentler, G Witman (Academic, San Diego), pp. 341–351 (1995). / Cilia and Flagella by Baron A T (1995)
10.2307/3001968
10.1038/356080a0
10.1242/jcs.93.1.63
10.1073/pnas.92.18.8493
10.1002/j.1460-2075.1989.tb08581.x
10.1016/0092-8674(89)90914-8
10.1172/JCI111527
10.1038/nm0196-72
10.1056/NEJM198809013190901
10.1016/0092-8674(94)90543-6
A Thor, D Yandell Molecular Pathology of Breast Carcinoma, eds J Harris, M Lippman, M Morrow, S Hellman (Lippincott-Raven, Philadelphia), pp. 445–454 (1996). / Molecular Pathology of Breast Carcinoma by Thor A (1996)
M W Lieberman, R M Lebovitz Neoplasia, eds I Damjanov, J Linder (Mosby, St. Louis) 1, 513–547 (1996). / Neoplasia by Lieberman M W (1996)
10.1038/386623a0
10.1016/0092-8674(92)90586-2
L Hartwell, T Weinert, L Kadyk, B Garvik Cell Cycle Checkpoints, Genomic Integrity, and Cancer, ed B Stillman (Cold Spring Harbor Lab. Press, Plainview, NY) LIX, 259–263 (1994). / Cell Cycle Checkpoints, Genomic Integrity, and Cancer by Hartwell L (1994)
10.1111/j.1524-4741.1995.tb00243.x
10.1016/0277-5379(87)90071-X
M Metcalf J Am Med Soc 84, 1140 (1925). / J Am Med Soc by Metcalf M (1925)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:19 p.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 9:48 a.m.)
Issued	27 years, 5 months ago (March 17, 1998)
Published	27 years, 5 months ago (March 17, 1998)
Published Online	27 years, 5 months ago (March 17, 1998)
Published Print	27 years, 5 months ago (March 17, 1998)

Funders 0

None

BibTeX

@article{Lingle_1998, title={Centrosome hypertrophy in human breast tumors: Implications for genomic stability and cell polarity}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.6.2950}, DOI={10.1073/pnas.95.6.2950}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lingle, Wilma L. and Lutz, Ward H. and Ingle, James N. and Maihle, Nita J. and Salisbury, Jeffrey L.}, year={1998}, month=mar, pages={2950–2955} }

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