DOI: 10.1073/pnas.95.6.2908. The <i>rpoB</i> mutants destabilizing initiation complexes at stringently controlled promoters behave like “stringent” RNA polymerases in <i>Escherichia coli</i>

The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like “stringent” RNA polymerases in Escherichia coli

10.1073/pnas.95.6.2908

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In Escherichia coli , stringently controlled genes are highly transcribed during rapid growth, but “turned off” under nutrient limiting conditions, a process called the stringent response. To understand how transcriptional initiation at these promoters is coordinately regulated, we analyzed the interactions between RNA polymerase (RNAP) (both wild type and mutants) and four stringently controlled promoters. Our results show that the interactions between RNAP and stringently controlled promoters are intrinsically unstable and can alternate between relatively stable and metastable states. The mutant RNAPs appear to specifically further weaken interactions with these promoters in vitro and behave like “stringent” RNAPs in the absence of the stringent response in vivo , constituting a novel class of mutant RNAPs. Consistently, these mutant RNAPs also activate the expression of other genes that normally require the response. We propose that the stability of initiation complexes is coupled to the transcription of stringently controlled promoters, and this unique feature coordinates the expression of genes positively and negatively regulated by the stringent response.

Bibliography

Zhou, Y. N., & Jin, D. J. (1998). The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like âstringentâ RNA polymerases in Escherichia coli. Proceedings of the National Academy of Sciences, 95(6), 2908â2913.

Authors 2

Yan Ning Zhou (first)
Ding Jun Jin (additional)

References 39 Referenced 193

M Cashel, D R Gentry, J V Hernandez, D Vinella Escherichia coli and Salmonella, Cellular and Molecular Biology, ed F C Neidhardt (Am. Soc. Microbiol., Washington, DC) 1, 1458–1496 (1996). / Escherichia coli and Salmonella, Cellular and Molecular Biology by Cashel M (1996)
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J H Miller Experiments in Molecular Genetics (Cold Spring Harbor Lab. Press, Plainview, NY, 1972). / Experiments in Molecular Genetics by Miller J H (1972)
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10.1111/j.1365-2958.1994.tb01336.x

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:15 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:21 a.m.)
Issued	27 years, 5 months ago (March 17, 1998)
Published	27 years, 5 months ago (March 17, 1998)
Published Online	27 years, 5 months ago (March 17, 1998)
Published Print	27 years, 5 months ago (March 17, 1998)

Funders 0

None

BibTeX

@article{Zhou_1998, title={The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like “stringent” RNA polymerases in Escherichia coli}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.6.2908}, DOI={10.1073/pnas.95.6.2908}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zhou, Yan Ning and Jin, Ding Jun}, year={1998}, month=mar, pages={2908–2913} }

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